6533b86ffe1ef96bd12cdd2d
RESEARCH PRODUCT
Effects of Parietaria judaica pollen extract on human microvascular endothelial cells
Giacomo De LeoSimona TavernaMarilisa BarrancaRiccardo AlessandroPaolo ColombaA. Flugysubject
AllergyNeutrophilsBiophysicsVascular Cell Adhesion Molecule-1parietaria judaicaBiologymedicine.disease_causeCell morphologyBiochemistryPermeabilitycell adhesion moleculesPollenCell AdhesionmedicineHumansProtease InhibitorsCytoskeletonLungMolecular BiologyCells CulturedActinPlant ExtractsCell adhesion moleculeInterleukin-8Cell BiologyAdhesionbiology.organism_classificationCapillariesCell biologyEndothelial stem cellParietariaImmunologyParietaria judaicaendothelial cellPollenEndothelium VascularE-Selectindescription
Abstract Pollinosis from Parietaria judaica is one of the main causes of allergy in the Mediterranean area. The present study is designed to assess if P. judaica pollens contain bioactive compounds able to elicit a functional response in endothelial cells. We have demonstrated that addition of pollen extract to human lung microvascular endothelial cells (HMVEC-L) induces a modification of cell morphology, actin cytoskeletal rearrangements and an increase in endothelial cell permeability. We further showed that the treatment of endothelial cells with pollen extract causes an increase of E-selectin and VCAM-1 protein levels as well as an increase of IL-8 production. The stimulation of cell–cell adhesion molecules was paralleled by a dose-dependent increase of adhesion of polymorphonuclear cells (PMNs) to HMVEC-L monolayer. Our results suggest for the first time that pollen affect directly endothelial cells (EC) modulating critical functions related to the inflammatory response.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-08-01 |