6533b86ffe1ef96bd12cdfa7
RESEARCH PRODUCT
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
Paula García-beldaPaula García-beldaFrédéric RelaixAntonio BernadLidwine TrouilhLidwine TrouilhLidwine TrouilhMacarena López-mayorgaPatricia García-parraSonia Alonso-martinAdolfo López De MunainM. GoicoecheaVéronique Anton-leberreVéronique Anton-leberreVéronique Anton-leberreJaime J. CarvajalAnder IzetaJosé Manuel García-verdugoJosé Manuel García-verdugoMarcos J. Araúzo-bravoAnder MatheuCarlos San JoséAna AiastuiNeia Naldaiz-gastesiDiego HerreroJ. Lacallesubject
0301 basic medicineWOUNDSCellular differentiation[SDV]Life Sciences [q-bio]CellCell Culture TechniquesMuscle DevelopmentMOUSEBiochemistryMicelcsh:QH301-705.5ComputingMilieux_MISCELLANEOUSlcsh:R5-920Gene Expression Regulation DevelopmentalPAX7 Transcription FactorCell Differentiation3. Good healthPanniculus carnosusCell biologyHaematopoiesisPhenotypemedicine.anatomical_structureMOUSE;TISSUE;REPAIR;WOUNDS;MYOGENESIS;EXPRESSION;SKIN;MODEL;SATELLITE CELLS;SKELETAL-MUSCLESKELETAL-MUSCLEMYF5Stem celllcsh:Medicine (General)EXPRESSIONSatellite Cells Skeletal MuscleBone Marrow CellsMice TransgenicBiologyArticleMYOGENESIS03 medical and health sciencesSATELLITE CELLSGeneticsmedicineAnimalsRegenerationCell LineageMuscle SkeletalPAX3 Transcription FactorCell ProliferationREPAIR[ SDV ] Life Sciences [q-bio]Cell growthCell BiologyMODEL030104 developmental biologylcsh:Biology (General)Cell cultureTISSUEImmunologyBiomarkersSKINDevelopmental Biologydescription
Summary The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5+, Pax3/Pax7+ cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-09-01 | Stem Cell Reports |