6533b870fe1ef96bd12cf149
RESEARCH PRODUCT
Engineering a 2D protein-DNA crystal.
Andrew J. TurberfieldJames C. MitchellJ. Robin HarrisCatherine Vénien-bryanDavid J. SherrattJonathan MaloHolger Willesubject
Molecular Sequence DataProtein dnaPlasma protein bindingCatalysislaw.inventionCrystalNucleic acid thermodynamicschemistry.chemical_compoundlawNanotechnologyBase sequenceCrystallizationOligonucleotide Array Sequence AnalysisDNA CruciformBase SequenceEscherichia coli ProteinsDNA HelicasesNucleic Acid HybridizationGeneral MedicineDNAGeneral ChemistryDNA-Binding ProteinsMicroscopy ElectronCrystallographychemistryBiochemistryCrystallizationDNAProtein Bindingdescription
(Figure Presented) Weaving with DNA: A DNA-binding protein was used to control the structure of a self-assembled 2D crystal. In the absence of protein, four oligonucleotides hybridize to form a Kagome lattice of interwoven double helices with p3 symmetry (see image). Addition of protein RuvA during assembly changes the symmetry and connectivity to give a DNA-protein crystal with an approximately square unit cell. © 2005 Wiley-VCH Verlag GmbH and Co. KGaA.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-05-13 | Angewandte Chemie (International ed. in English) |