6533b870fe1ef96bd12cf367
RESEARCH PRODUCT
Studies on DNA interaction of organotin(IV) complexes of meso-tetra(4-sulfonatophenyl)porphine that show cellular activity.
Tiziana FioreClaudia PelleritoTarita BiverStefania FigucciaSonia MontanaroSabriye Aydinoglusubject
Steric effectsCellular activityOrganometallic compounds External binding Negative porphyrins Aggregation ViscosityNegative porphyrinsPorphyrinsStereochemistryBinding energyDna interactionOrganometallic compounds010402 general chemistry01 natural sciencesMedicinal chemistryBiochemistryInorganic Chemistrychemistry.chemical_compoundAggregationmetal complexequilibrium constantDNA conformation changescomplex formationOrganotin CompoundsHumansDNA bindingEquilibrium constantGroup 2 organometallic chemistrybiology010405 organic chemistryViscosityDNAbiology.organism_classificationExternal binding0104 chemical sciencesAggregation External binding Negative porphyrins Organometallic compounds Viscosity Biochemistry Inorganic Chemistry metal complex equilibrium constant DNA conformation changes DNA binding complex formationchemistrySettore CHIM/03 - Chimica Generale E InorganicaA549 CellsTetraNucleic Acid ConformationDNAdescription
PubMedID: 27393277 The interaction of the diorgano- and triorganotin(IV) derivatives of meso-tetra-(4-sulfonatophenyl)porphine (Me2Sn)2TPPS, (Bu2Sn)2TPPS, (Me3Sn)4TPPS and (Bu3Sn)4TPPS to natural DNA was analysed (together with free meso-tetra-(4-sulfonatophenyl)porphine (TPPS4 -) for comparison purposes). Particular attention was paid to (Bu3Sn)4TPPS, a species that shows significant cellular action. Preliminary tests were done on the solution properties of the organotin(IV) compounds (pKA and possible self-aggregation). Spectrophotometric and spectrofluorometric experiments showed that all the investigated organotin(IV) derivatives strongly interact with DNA, the binding energy depending on the dye steric hindrance. In all cases experimental data concur in indicating that external binding mode prevails. Interestingly, fluorescence quenching and viscosity experiments show that the Bu-containing species, and in particular (Bu3Sn)4TPPS, are able to noticeably alter the DNA conformation. © 2016 Elsevier Inc. Università degli Studi di Palermo “la Caixa” Foundation: OSLC-2012-007 PRA_2015_0038 Ministerio de Economía, Industria y Competitividad, Gobierno de España: MINECO CTQ2014-58812-C2-2-R The financial support by PRA 2015 of the University of Pisa , project PRA_2015_0038 , by Obra Social “la Caixa” , project OSLC-2012-007 , by the Ministerio de Economia y Competividad - Gobierno de España , project MINECO CTQ2014-58812-C2-2-R , and by Università degli Studi di Palermo Grant ORPA07SLXE are gratefully acknowledged. Appendix A
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-01-01 | Journal of inorganic biochemistry |