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RESEARCH PRODUCT
Profiling of RNA modifications by multiplexed stable isotope labelling
René F. KettingDavid RosenkranzJennifer NeumannStefanie KellnerDirk SchneiderHans ZischlerSvetlana LebedevaMark Helmsubject
ChemistryStable isotope ratioMetals and AlloysRNASaccharomyces cerevisiaeGeneral ChemistryTandem mass spectrometryCatalysisYeastSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsBiochemistryTandem Mass SpectrometryIsotope LabelingLabellingEscherichia coliMaterials ChemistryCeramics and CompositesRNAChromatography Liquiddescription
The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-02-26 | Chemical Communications |