6533b870fe1ef96bd12cfbd2
RESEARCH PRODUCT
Cytogenetic manifestations associated with the reversion, by gene amplification, at the HGPRT locus in V79 Chinese hamster cells.
R. RandazzoG. SciandrelloC. AgneseA. MaddalenaP. CavolinaA. Di Leonardosubject
GeneticsGene Rearrangementmedicine.medical_specialtyHypoxanthine PhosphoribosyltransferaseCytogeneticsReversionGene AmplificationChromosomeHamsterKaryotypeGeneral MedicineGene rearrangementBiologybiology.organism_classificationMolecular biologyChinese hamsterCell LineCricetinaeMutationGeneticsmedicineAnimalsX chromosomedescription
SummarySome HGPRT spontaneous revertants were isolated from a mutant line (E2) of V79 Chinese hamster cells and phenotypically characterized. Dot–Blot hybridization with a32P-Iabelled HGPRT probe revealed an increase in the number of HGPRT sequences in some of these revertants, suggesting the occurrence of gene amplification. Cytogenetic analysis performed in three of these revertants showed a characteristic abnormally banding region (ABR) on the elongated p arm of theXchromosome.In Situhybridization in one revertant (RHE2) showed that the amplified sequences reside on the p+arm of theXchromsome in two different localizations. Because of the very probable clonal origin of the revertant, these features indicate that the amplified sequences might rearrange after their integration into the chromosome.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-06-01 | Genetical research |