6533b870fe1ef96bd12cfc8b
RESEARCH PRODUCT
Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly.
Calogero CarusoGiuseppina Colonna-romanoMatteo BulatiAdriana MartoranaDomenico LioSilvio BuffaCandoregiuseppinasubject
AdultAgingChemokine receptorNaive B cellB-cell receptorB-Lymphocyte Subsetschemical and pharmacologic phenomenaBiologyCXCR3BiochemistryGranzymesEndocrinologyImmune systemElderlyIL-21GeneticsHumansSettore MED/05 - Patologia ClinicaL-SelectinMemory B cellMolecular BiologyAgedAged 80 and overReceptors CXCRSettore MED/04 - Patologia GeneraleB lymphocyteGranzyme BInterleukinshemic and immune systemsImmunoglobulin DCell BiologyInflamm-agingTumor Necrosis Factor Receptor Superfamily Member 7B-1 cellGranzyme BImmunosurveillancePhenotypeImmunoglobulin GImmunologydescription
The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG(+)IgD(-)CD27(-), double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naïve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naïve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, naïve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of "inflamm-aging". In particular IgG(+)IgD(-)CD27(-) DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-09-30 |