6533b870fe1ef96bd12cfc93

RESEARCH PRODUCT

Stabilization and detection of hydrophylloquinone as di-O-methyl derivative

Antonio Doménech-carbóGerardo Cebrián-torrejónAlejandro M. KatzinMarcilio M. De MoraesExequiel O. J. PortaMassuo J. KatoLydia F. YamaguchiRodrigo A.c. Sussmann

subject

0301 basic medicineClinical BiochemistryNanotechnologyElectrochemistryBiochemistryHigh-performance liquid chromatographyRedoxMethylationHydrophylloquinoneAnalytical ChemistryELECTROCHEMISTRY03 medical and health scienceschemistry.chemical_compoundHYDROPHYLLOQUINONEElectrochemistryRedox activeChromatography High Pressure LiquidChromatographyREDOXMethyl derivativeCiencias QuímicasCROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIACell BiologyGeneral MedicineVitamin K 1Combinatorial chemistryElectron transport chainNaphthoquinoneDI-O-METHYL DERIVATIVE030104 developmental biologychemistryPHYLLOQUINONEQuímica AnalíticaHPLCCIENCIAS NATURALES Y EXACTAS

description

Phylloquinone is a redox active naphthoquinone involved in electron transport in plants. The function of this reduced form remains unclear due to its instability, which has precluded detection. Herein, a simple method that permits the stabilization of the reduced form of phylloquinone by di-O-methylation and HPLC detection is described. Fil: Sussmann, Rodrigo A. C.. Universidade de Sao Paulo; Brasil Fil: de Moraes, Marcilio M.. Universidade de Sao Paulo; Brasil Fil: Cebrián Torrejón, Gerardo. Universidad de Valencia; España. Universidade de Sao Paulo; Brasil Fil: Porta, Exequiel Oscar Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Doménech Carbó, Antonio. Universidad de Valencia; España Fil: Yamaguchi, Lydia F.. Universidade de Sao Paulo; Brasil Fil: Katzin, Alejandro M.. Universidade de Sao Paulo; Brasil Fil: Kato, Massuo J.. Universidade de Sao Paulo; Brasil

10.1016/j.jchromb.2016.09.011