6533b870fe1ef96bd12d057b

RESEARCH PRODUCT

Mass spectrometry strategies for mycotoxins analysis in European beers

Josep RubertJordi MañesCarla SolerKevin J. JamesR Marı́n

subject

Ochratoxin ATriple quadrupoleChromatographyRelative standard deviationBeerRepeatabilityMycotoxinsMass spectrometryOrbitrapUltra high resolutionOrbitraplaw.inventionTriple quadrupole mass spectrometerchemistry.chemical_compoundchemistrylawSPEMycotoxinFood ScienceBiotechnology

description

In this work, an existent solid-phase extraction (SPE) procedure was used to study the occurrence of mycotoxins in different European beers. HPLC-QqQ-MS/MS and ultra high resolution mass analyser have been optimized for the analysis of 18 mycotoxins: the methods were validated according to the EU Commission Decision 2002/657/EC guidelines. In this sense, matrix-matched calibration was performed for each type of beer, obtaining an effective quantification. The recoveries ranged from 63 to 91% and repeatability and reproducibility expressed as relative standard deviation (RSD%) were lower than 17%. On one hand, HPLC-LTQ-Orbitrap ® was used for unambiguous identification of target mycotoxins, as well as for screening non-target mycotoxins, such as enniatins, fusaproliferin and deoxynivalenol-3-glucoside. On the other hand, the quantification was carried out using HPLC-QqQ-MS/MS instrument. At the end, Ochratoxin A, fumonisins, HT-2 and T-2 toxins were detected and quantified in European beers. Moreover, pale lager beer showed higher mycotoxin incidence than the other studied beers.

yearjournalcountryeditionlanguage
2013-03-01
10.1016/j.foodcont.2012.06.035https://research.wur.nl/en/publications/mass-spectrometry-strategies-for-mycotoxins-analysis-in-european-