Penicillium lanoso-viride AMP dezamināzes attīrīšana, izmantojot kolonnu hromatogrāfiju

6533b871fe1ef96bd12d13a7

RESEARCH PRODUCT

Penicillium lanoso-viride AMP dezamināzes attīrīšana, izmantojot kolonnu hromatogrāfiju

Jānis Miezītis

subject

AMP sefaroze AMP dezamināze DEAE celuloze Bioloģija proteīnu attīrīšana kolonnu hromatogrāfija

description

Darba mērķis bija iegūt pēc iespējas attīrītākas AMP dezamināzes frakcijas no mikroskopiskās sēnes Penicillium lanoso-viride glikoproteīnu preparātiem, izmantojot kolonnu hromatogrāfijas metodes, un raksturot iegūtās frakcijas. Mērķa sasniegšanai tika veikta hromatogrāfija DEAE celulozes un AMP sefarozes hromatogrāfijas kolonnās, kā arī attīrīšana, inkubējot proteīnu preparātus mēģenēs, kas pildītas ar DEAE celulozi. Pēc attīrīšanas ar DEAE celulozes hromatogrāfijas kolonnu tika iegūta aptuveni 3.7 reizes attīrītāka AMP dezamināzes frakcija, bet ar AMP sefarozes hromatogrāfijas metodi izdevās iegūt AMP dezamināzi ar attīrīšanas pakāpi 2.37 reizes. Ar DEAE celulozi pildītās mēģenēs AMP dezamināzes proteīnu preparātus izdevās attīrīt 2.63 reizes.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/34088

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