6533b871fe1ef96bd12d187b

RESEARCH PRODUCT

Comparative study of reflectance cells for PLS-FTIR determination of sugars in soft drinks

Salvador GarriguesM. De La GuardiaF. J. Rambla

subject

Detection limitAbsorbancechemistry.chemical_compoundSucrosechemistryAttenuated total reflectionPartial least squares regressionAnalytical chemistryFructoseSugarBiochemistryQuantitative analysis (chemistry)

description

A comparative study has been carried out between a horizontal Attenuated Total Reflectance (h-ATR) cell and a Cylindrical Internal Reflection (CIR) cell (CIRCLE cell). Both cells were employed for the determination of glucose, fructose, sucrose and total sugar in soft drinks and fruit juices using absorbance measurements at two resolution values (4 and 8 cm–1). Data were processed by Partial Least Squares regression (PLS). Using a resolution of 4 cm–1, mean relative errors of prediction from 0.6% to 1.3% were obtained using the horizontal-ATR and from 0.6% to 2.8% using the CIRCLE cell resulting in a limit of detection from 0.03% in the case of sucrose to 0.21% for total sugar for the h-ATR and from 0.06% for sucrose to 0.13% for glucose using the CIRCLE cell. Both cells provided appropriate figures of merit, but the analytical sensitivity obtained using the h-ATR cell was three times higher than that obtained using the CIRCLE cell.

yearjournalcountryeditionlanguage
1998-09-04Fresenius' Journal of Analytical Chemistry
https://doi.org/10.1007/s002160051046