6533b871fe1ef96bd12d1987
RESEARCH PRODUCT
Characterization of antigenic epitopes of potato virus Y.
Aarne KurppaKatariina PaananenMaija Vihinen-rantaMatti Vuentosubject
Peptide BiosynthesisAntigenicity030303 biophysicsMolecular Sequence DataBiophysicsAntibodies ViralBiochemistryEpitopeVirusProtein Structure SecondaryPlant Viruses03 medical and health sciencesEpitopesCapsidAntigenStructural BiologyAnimalsAmino Acid SequenceMolecular BiologyProtein secondary structure030304 developmental biologyAntiserum0303 health sciencesbiologyPotyvirusbiology.organism_classificationVirologyMolecular biology3. Good healthPotato virus YRabbitsPeptidesdescription
Immunochemical analysis of overlapping synthetic hexapeptides covering the entire length of the coat protein of potato virus Y (PVY) revealed immunodominant regions both at the N-terminal and at the C-terminal end of the coat protein. Immunization of rabbits with synthetic peptides representing N- and C-terminal regions of the coat protein resulted in production of antibodies that reacted with PVY. Antigenicity of PVY peptides was found to correlate with predicted beta turns, with hydrophilicity and with predicted chain flexibility. Characterization of the immunochemical properties of PVY will facilitate the development of detection methods for potyviruses.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-03-01 | Biochimica et biophysica acta |