6533b871fe1ef96bd12d1ab8

RESEARCH PRODUCT

A first comparative map of copy number variations in the sheep genome.

Vincenzo RussoRita CasadioPier Luigi MartelliFrancesca BerettiFrancesca BerettiStefania Dall'olioM. OccidenteDonato MatassinoBaldassare PortolanoLuca FontanesiMario P. Colombo

subject

Ovis ariesDNA Copy Number VariationsRuminantSheep breedsaCGH; Comparative map; Copy number variation; Ovis aries; Ruminants; Sheep breedsGenomicsOvis arieBiologyGenomeChromosomesSettore AGR/17 - Zootecnica Generale E Miglioramento Genetico03 medical and health sciencesaCGHChromosome regionsGeneticsAnimalsCopy-number variationGene030304 developmental biologyOligonucleotide Array Sequence AnalysisGeneticsCOMPARATIVE MAPPING0303 health sciencesComparative Genomic HybridizationGenomeSheepCopy number variation0402 animal and dairy scienceComparative Genome HybridizationChromosome Mapping04 agricultural and veterinary sciencesRuminantsbiology.organism_classification040201 dairy & animal scienceBovine genomeSardaCattleComparative map

description

article i nfo We carried out a cross species cattle-sheep array comparative genome hybridization experiment to identify copy number variations (CNVs) in the sheep genome analysing ewes of Italian dairy or dual-purpose breeds (Bagnolese, Comisana, Laticauda, Massese, Sarda, and Valle del Belice) using a tiling oligonucleotide array with ~385,000 probes designed on the bovine genome. We identified 135 CNV regions (CNVRs; 24 reported in more than one animal) covering ~10.5 Mb of the virtual sheep genome referred to the bovine genome (0.398%) with a mean and a median equal to 77.6 and 55.9 kb, respectively. A comparative analysis between the identified sheep CNVRs and those reported in cattle and goat genomes indicated that overlaps between sheep and both other species CNVRs are highly significant (Pb0.0001), suggesting that several chromosome regions might contain recurrent interspecies CNVRs. Many sheep CNVRs include genes with important biological functions. Further studies are needed to evaluate their functional relevance.

yearjournalcountryeditionlanguage
2011-03-01Genomics
10.1016/j.ygeno.2010.11.005https://pubmed.ncbi.nlm.nih.gov/21111040