6533b871fe1ef96bd12d1ae9

RESEARCH PRODUCT

Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG), a reliable oxidative stress marker in hypertension

Jorge Jiménez-almazánAmparo SalvadorMarta FandosM. Carmen TormosOlga EspinosaJosep RedonFelipe-javier ChavesGuillermo-t. SáezSonia ClapésAntonio Iradi

subject

AdultMalemedicine.medical_specialtyDNA damageUrinary systemUrinemedicine.disease_causeDNA MitochondrialBiochemistryPeripheral blood mononuclear cellchemistry.chemical_compoundInternal medicinemedicineHumansDeoxyguanosineChromatography High Pressure LiquidCell NucleusGlutathione DisulfideDeoxyguanosineGeneral MedicineGlutathioneGlutathioneOxidative StressEndocrinologychemistryBiochemistry8-Hydroxy-2'-DeoxyguanosineHypertensionGlutathione disulfideFemaleBiomarkersOxidative stress

description

The potential use of oxidative stress products as disease markers and progression is an important aspect of biomedical research. In the present study, the quantification of urine 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) concentration has been used to express the oxidation status of hypertensive subjects. 8-oxo-dG has been simultaneously isolated and assayed in nuclear (nDNA) and mitochondrial DNA (mtDNA). In addition, oxidative stress of mononuclear cells has been estimated by means of GSH and GSSG levels and GSSG/GSH ratio in hypertensive subjects before and after antihypertensive treatment. It is shown that oxidative stress decreases significantly in hypertensive patients after treatment the effect being accompanied by reduction of their blood pressure. A significant correlation is observed comparing the yield of urine 8-oxo-dG and that isolated from mitochondria DNA. Moreover, urinary excretion of 8-oxo-dG also correlates with the GSSG/GSH ratio of cells.urine 8-oxo-dG assay is a good marker for monitoring oxidative stress changes in hypertensives.

yearjournalcountryeditionlanguage
2007-04-25Free Radical Research
https://doi.org/10.1080/10715760601164050