6533b871fe1ef96bd12d1c7c

RESEARCH PRODUCT

Pulsed field gel electrophoresis and genome size estimates

Rosa AlduinaAnnalisa Pisciotta

subject

Genome sizeDNA BacterialMaterials scienceChromosomes ArchaealSettore BIO/19 - Microbiologia GeneraleGenomePlasmidchemistry.chemical_compoundPlasmidGeneticGenome ArchaealElectric fieldPulsed-field gel electrophoresisGenome sizeMolecular BiologyElectrophoresis Agar GelBase CompositionStrain (chemistry)BacteriaMulti-repliconMedicine (all)Physical Chromosome Mappingfood and beveragesChromosomes BacterialPhysical Chromosome MappingArchaeaElectrophoresis Gel Pulsed-FieldDNA ArchaealchemistryMegaplasmidBiological systemDNAGenome BacterialGenome topology

description

Pulsed field gel electrophoresis (PFGE) is a quick and reliable procedure to resolve DNA molecules larger than 30 kb by applying an electric field that periodically changes direction. This technique can be used to estimate genome size of a microorganism, to reveal if a genome is circular or linear, to indicate the presence of megaplasmids, and to show if a strain contains only one or more chromosomes.

yearjournalcountryeditionlanguage
2015-01-01
10.1007/978-1-4939-1720-4_1http://hdl.handle.net/10447/253753