6533b871fe1ef96bd12d2404
RESEARCH PRODUCT
Nanomolar pyrophosphate detection in water and in a self-assembled hydrogel of a simple terpyridine-Zn2+ complex.
Varpu MarjomäkiBiswa Nath GhoshSandip BhowmikKari Rissanensubject
PyridinesConfocalAnalytical chemistryBiochemistryPyrophosphateCatalysischemistry.chemical_compoundColloid and Surface ChemistryLimit of DetectionMicroscopyFluorescence microscopeta116Detection limitta1182WaterHydrogelsGeneral ChemistryFluorescenceDiphosphatesZincchemistryMicroscopy FluorescenceSelf-healing hydrogelsSpectrophotometry UltravioletTerpyridineNuclear chemistrydescription
A simple terpyridine-Zn(II) complex is shown to act as an efficient and highly selective fluorescent sensor for pyrophosphate in water at physiological pH. The sensor complex showed an unprecedented fluorescence response (∼500 fold increase) and a record nanomolar sensitivity (detectable fluorescent response at 20 nM and LOD ∼ 0.8 nM). It has successfully been used to stain and record confocal fluorescence microscopy images of HeLa cells. Moreover, the complex was found to self-assemble into a hydrogel which was subsequently used to coat disposable paper strips for easy, low-cost detection of pyrophosphate.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-02-06 | Journal of the American Chemical Society |