6533b871fe1ef96bd12d242f
RESEARCH PRODUCT
Influence of hydrocortisone on chick embryo retina development
Giuseppina D'anconaMichela GiulianoGiovanni TesoriereGennaro TaibiRenza Ventosubject
medicine.medical_specialtyThymidine kinase activityanimal structuresHydrocortisoneInfluence of hydrocortisonemedicine.medical_treatmentChick EmbryoAdrenocorticotropic hormoneBiologyIn ovoThymidine KinaseBiochemistryRetinaCellular and Molecular Neurosciencechemistry.chemical_compoundAdrenocorticotropic HormoneInternal medicinemedicineAnimalsHydrocortisoneMetyraponeRetinalMetyraponeSteroid hormoneEndocrinologychemistryThymidine kinaseembryonic structuresmedicine.drugdescription
Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-06-01 |