6533b871fe1ef96bd12d261f

RESEARCH PRODUCT

Biosynthesis and transformation of 20α 21-dihydroxycholesterol by rat adrenal preparations

G. DiedrichsenS.h.e. KaufmannK. SinterhaufD. Lommer

subject

Malemedicine.drug_classStereochemistryBiology17-alpha-HydroxypregnenoloneBiochemistrychemistry.chemical_compoundEndocrinologyBiosynthesisMicrosomesAdrenal Glandspolycyclic compoundsmedicineAnimalsCholesterol Side-Chain Cleavage EnzymeCholesterol side-chain cleavage enzymeHydroxycholesterolsMitochondriaRatsTransformation (genetics)BiochemistrychemistryAlternative complement pathwayPregnenoloneMicrosomeCorticosteroidlipids (amino acids peptides and proteins)NADPmedicine.drug

description

Abstract The biosynthesis of [ 3 H]-20α, 21 dihydroxycholestderol from [ 3 H]-20α-hydroxycholesterol and its transformation to [ 3 H]-21-hydroxypregnenolone by rat adrenal preparations has been demonstrated. 20α-Hydroxycholesterol was transformed to 20α, 21-dihydroxycholesterol by microsomal preparations in the presence of NADPH and 20α-21-dihydroxycholesterol was metabolized to 21-hydroxypregnenolone by mitochondrial preparations in the presence of a NADPH-generating-system. Comparison of the Michaelis-Menten-Kinetics of the steps “20α, 21-dihydroxycholesterol → 21-hydroxycholesterol” and “20α-hydroxycholesterol → pregnenolone” revealed that both compounds behaved as analogue substrates of the desmolase complex. The data are taken as further evidence for the participation of an alternative pathway via the sequence “21-hydroxysterol → 21-hydroxypregnenolone → deoxycorticosterone” in rat adrenal corticosteroid biosynthesis.

yearjournalcountryeditionlanguage
1979-06-01Journal of Steroid Biochemistry
https://doi.org/10.1016/0022-4731(79)90517-x