6533b872fe1ef96bd12d37a6
RESEARCH PRODUCT
Studies on the metabolism of mitragynine, the main alkaloid of the herbal drug Kratom, in rat and human urine using liquid chromatography-linear ion trap mass spectrometry
Anika A. PhilippJidapha KanogsunthornratHans H. MaurerSiegfried W. ZoerntleinOliver N. KleinDirk K. Wissenbachsubject
ChromatographyIndole alkaloidbiologyAlkaloidMitragyna speciosaUrineMetabolismMass spectrometrybiology.organism_classificationchemistry.chemical_compoundchemistryMitragynine7-HydroxymitragynineSpectroscopydescription
Mitragynine (MG) is an indole alkaloid of the Thai medicinal plant Mitragyna speciosa (Kratom in Thai) and reported to have opioid agonistic properties. Because of its stimulant and euphoric effects, Kratom is used as a herbal drug of abuse. The aim of the presented study is to identify the phase I and II metabolites of MG in rat and human urine after solid-phase extraction (SPE) using liquid chromatography-linear ion trap mass spectrometry providing detailed structure information in the MSn mode particularly with high resolution. The seven identified phase I metabolites indicated that MG was metabolized by hydrolysis of the methylester in position 16, O-demethylation of the 9-methoxy group and of the 17-methoxy group, followed, via the intermediate aldehydes, by oxidation to carboxylic acids or reduction to alcohols and combinations of some steps. In rats, four metabolites were additionally conjugated to glucuronides and one to sulfate, but in humans, three metabolites to glucuronides and three to sulfates.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-06-17 | Journal of Mass Spectrometry |