6533b872fe1ef96bd12d40b5
RESEARCH PRODUCT
PCR-Amplified cDNA Probes for Verification of Differentially Expressed Genes
Ralf RossAngelika B. Reske-kunzKatja Kumpfsubject
Differential displayCell typeBiologyMolecular biologyGeneral Biochemistry Genetics and Molecular Biologylaw.inventionNucleic acid thermodynamicslawComplementary DNAGene expressionMolecular probeGenePolymerase chain reactionBiotechnologydescription
Differential display has proven to be a powerful technique for the detection and isolation of differentially expressed genes. By generating reproducible cDNA expression patterns, it is possible to compare gene expression by two or more cell types, developmental stages or tissues and to isolate as yet unknown differentially expressed genes. A sensitive method is necessary to verify the differential expression of the isolated cDNAs. Here we describe the use of adaptor-ligated, PCR-amplified total cDNA of the two cell types compared as a probe for Southern hybridizations with the isolated cDNAs.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-05-01 | BioTechniques |