6533b872fe1ef96bd12d40cf
RESEARCH PRODUCT
Small airways in in sedentary and endurance-trained dystrophic (mdx) mice
Francesca RappaMonica FrinchiMaria R. BonsignoreFrancesco CappelloGiuseppa MudòNatale BelluardoGiuseppe Moricisubject
medicine.medical_specialtyTUNEL assaybiologybusiness.industrySmall airwaysAnatomyPeriodic acid–Schiff stainStainingProliferating cell nuclear antigenEndocrinologyEndurance trainingApoptosisInternal medicinebiology.proteinMedicineImmunohistochemistrybusinessdescription
The effects of mild endurance exercise training on the small airways in mdx mice are unknown. We compared epithelial thickness and turnover, apoptosis, and stress marker expression in small airways of mdx mice and wild-type (WT) controls, at rest and during exercise training. Mdx and WT mice were randomly assigned to sedentary (mdx-S, n=17; WT-S, n=19) or trained (mdx-EX, n=14; WT-EX, n=16) groups. Low-intensity endurance training (running on a wheel) was done 5 d/wk for 6 wk at progressively increasing speed (rpm from 16 to 24) and time (15 min to 1 h). Lungs were processed for light microscopy and periodic acid Schiff (PAS) staining. Hsp60 and PCNA were quantified by immunohistochemistry. Apoptosis was assessed by TUNEL. Bronchial epithelial thickness decreased over time in WT mice irrespective of training (linear regression for time trends: WT-S: R 2 =0.43, r= -0.65; WT-EX: R 2 =0.68, r= -0.82, p 2 =0.44, r=-0.66, p=0.01). In conclusion, bronchiolar epithelium in mdx mice is poor of goblet cells, and progressively deteriorates over time possibly because of loss of stress-related protective mechanism. Mild training did not cause any additional damage.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-09-01 | 4.1 Clinical respiratory physiology, exercise and functional imaging |