6533b872fe1ef96bd12d4318
RESEARCH PRODUCT
Isolation and Characterization of an Fe(III)-Chelating Compound Produced by Pseudomonas syringae
José E. Pérez-ortínVicente TorderaLuis Enrique Nores TorresJosé Beltránsubject
SiderophoreEcologyStereochemistryBiologybiology.organism_classificationApplied Microbiology and BiotechnologyFluorescenceMicrobiologyPigmentColumn chromatographyvisual_artPseudomonadalesPseudomonas syringaevisual_art.visual_art_mediumMoietyChelationMicroorganism-Plant InteractionsFood ScienceBiotechnologydescription
The phytopathogenic bacterium Pseudomonas syringae produces a fluorescent pigment when it is grown in iron-deficient media. This pigment forms a very stable Fe(III) complex that was purified in this form by using a novel procedure based on ultrafiltration and column chromatography. The Fe(III) complex has a molecular weight of 1,100 and contains 1 mol of Fe(III). The pigment is composed of an amino acid moiety with three threonines, three serines, one lysine, δ- N -hydroxyornithine, and a quinoline-type fluorescent chromophore. These features and its stability constant (in the range of 10 32 ) suggest that the fluorescent pigment of P. syringae is related to the siderophores produced by another Pseudomonas species.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1986-07-01 | Applied and Environmental Microbiology |