6533b872fe1ef96bd12d4365

RESEARCH PRODUCT

rRNA probing of chromosomal DNA of epidemic and sporadic isolates of Salmonella enterica subsp. Enterica serovar kottbus from Northern and Southern Italy

Caterina MamminaA. NastasiMirella PontelloM. CorbelliniVillafrate Mr

subject

DNA BacterialSerotypeEpidemiologyPopulationmedicine.disease_causeDisease OutbreaksMicrobiologySalmonellaEscherichia coliHumansMedicineGeneEscherichia colibiologybusiness.industryGenetic VariationNucleic Acid HybridizationOutbreakRNA ProbesRibosomal RNAbiology.organism_classificationDNA FingerprintingEnterobacteriaceaeBlotting SouthernItalyRNA RibosomalSalmonella entericaSalmonella Food PoisoningSalmonella enterica subsp. entericabusiness

description

Fifty-two strains of Salmonella enterica subsp. enterica serovar Kottbus, identified at the Centres of Enterobacteriaceae of Northern and Southern Italy, were investigated by molecular genetic methods. Thirteen isolates were recovered during two food-poisoning outbreaks that occurred in May 1987 in Lombardy. The rDNA gene restriction patterns, obtained by probing endonuclease cleaved chromosomal DNA with photobiotin labeled Escherichia coli rRNA, revealed some heterogeneity among strains isolated from Southern Italy, whereas Northern Italy isolates exhibited virtually identical banding patterns.

https://doi.org/10.1007/bf00151716