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RESEARCH PRODUCT
Analysis of cannabinoids by liquid chromatography–mass spectrometry in milk, liver and hemp seed to ensure food safety
María Jesús Andrés-costaYolanda PicoÚRsula EscriváVicente Andreusubject
Hemp seedFood Safety010501 environmental sciences01 natural sciencesMass SpectrometryAnalytical ChemistryFood safetyLC–MS/MSLiquid chromatography–mass spectrometryLc ms msAnimalsSolid phase extraction0105 earth and related environmental sciencesSolid-phase extractionChromatographyChemistrybusiness.industryCannabinoidsSolid Phase Extraction010401 analytical chemistryExtraction (chemistry)General MedicineFood safety0104 chemical sciencesMilkLiverSeedsbusinessDecision limitChromatography LiquidFood ScienceFood of animal origindescription
A method for determining cannabinoids, Δ9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Δ9-THC (THC-COOH) and 11-hidroxy-Δ9-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60 mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) ranged from 3.10–10.5 ng g−1 and 3.52–11.5 ng g−1, the recoveries were 76–118% and matrix effect ranged from −17.8% to 19.9% in the three matrices studied. The method was applied to food samples obtaining positive results for THC in hemp seeds (average 0.82 μg g−1) and three brands of junior formula milk at concentrations from 4.76 to 56.11 ng g−1. The developed method was suitable achieving identification and quantification of cannabinoids in food matrices.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-08-01 |