6533b873fe1ef96bd12d4cbb

RESEARCH PRODUCT

Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils

David P. H. LejonPierre-alain MaronVirginie NowakMagali MaitreLionel RanjardAnne Mercier

subject

Soil testRELATION SOL MICROORGANISMEMicrobial metabolismBACTERIAL COMMUNITYCARTOGRAPHIE GENETIQUEBiologycomplex mixturesMicrobiologyMicrobiologySoilBacterial ProteinsPeptide mass fingerprintingBotanyMolecular BiologySoil MicrobiologyBacteriaInoculationPROTEIN FINGERPRINTINGGeneral MedicineDNA Fingerprinting[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMicrobial population biologyDNA profilingSoil waterElectrophoresis Polyacrylamide GelMETAPROTEOMESoil microbiology

description

The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and soil parameters, with pH, clay and CaCO(3) contents being the most discriminating factors.

yearjournalcountryeditionlanguage
2007-12-17Research in Microbiology
https://doi.org/10.1016/j.resmic.2008.03.004