6533b873fe1ef96bd12d4d7a
RESEARCH PRODUCT
Chromatographic determination of caffeine in pharmaceutical formulations using micellar mobile phases
Salvador SagradoI. Perez-martinezM.j. Medina-hernándezsubject
ChromatographyChemistrySodiumOrganic ChemistryClinical Biochemistrychemistry.chemical_elementRepeatabilityBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryPropanolchemistry.chemical_compoundMicellar liquid chromatographySpherisorb octadecylsilaneCaffeineQuantitative analysis (chemistry)description
An HPLC procedure is described for the determination of caffeine in pharmaceutical preparations. A Spherisorb octadecylsilane ODS-2 C18 analytical column and spectrophotometric detection at 273 nm were used. The chromatographic behaviour of caffeine with different micellar eluents containing sodium dodecyl sulphate (SDS) is described. The determination of caffeine in pharmaceutical preparations was performed by use of a mobile phase containing 0.05 M sodium dodecylsulphate (SDS) and 1.5% propanol at pH7. At a 6.0 μg mL−1 concentration level the peak area and peak height repeatability were 2.6 and 2.4%, respectively. The application of the proposed method to the analysis of five pharmaceutical formulations, using peak heights as the dependent variable, gave recoveries between 85 and 104% of the values declared by the manufacturers. The proposed procedure for the determination of caffeine is rapid (15 min per sample), reliable and free from interferences.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-08-01 |