6533b873fe1ef96bd12d4e0b

RESEARCH PRODUCT

Characterization of the CtsR stress response regulon in Lactobacillus plantarum.

Stéphanie WeidmannGiuseppe SpanoAnna GalloneTarek MsadekMichael CollinsDaniela FioccoPascal HolsVittorio CapozziJean GuzzoAurélie Rieu

subject

OperonMutantBiology[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyMicroscopy Atomic ForceMicrobiologyRegulonMicrobiologylaw.invention03 medical and health sciencesBacterial ProteinslawGene RegulationPromoter Regions GeneticMolecular BiologyGeneHeat-Shock Proteins030304 developmental biologyGenetics0303 health sciences030306 microbiologyReverse Transcriptase Polymerase Chain ReactionTemperaturePromoterGene Expression Regulation Bacterialbiology.organism_classificationPhenotype[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyRepressor ProteinsRegulonRecombinant DNALactobacillus plantarumProtein BindingLactobacillus plantarum

description

ABSTRACT Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the Δ ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR - clpC operon and hsp1 .

10.1128/jb.01122-09https://hal.science/hal-00450675