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RESEARCH PRODUCT
Quantitative determination of moniliformin in vegetable foods and feeds
Christa JansenKlaus Dosesubject
Detection limitChromatographyClinical BiochemistryExtraction (chemistry)General MedicineHigh-performance liquid chromatographyThin-layer chromatographyAnalytical Chemistrychemistry.chemical_compoundchemistryReagentGeneral Materials ScienceDerivatizationMycotoxinMoniliformindescription
A suitable and simple method for the quantitative determination of moniliformin in vegetable foods and feeds is described. The mycotoxin was extracted by Soxhlet extraction with methanol from mouldy maize, rice, rye, oats, wheat and barley samples. Moniliformin was determined by thin-layer chromatography (TLC) using N-methylbenzthiazolon-2-hydrazone (MBTH) as a new derivatization reagent for this mycotoxin. The moniliformin derivative was assayed at 518 nm. Quantification could be performed after calibration. A linear relationship between mycotoxin amount and peak area was found from 100 to 400 ng/spot. The detection limit is 75 ng/spot.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1984-01-01 | Fresenius' Zeitschrift für analytische Chemie |