6533b873fe1ef96bd12d57d3
RESEARCH PRODUCT
A factor derived from chick embryo retina which inhibits DNA synthesis of retina itself.
Giovanni TesoriereMichela GiulianoRenza VentoGennaro TaibiGiuseppe Calvarusosubject
animal structuresChick EmbryoBiologyBiochemistryChromatography AffinityRetinaCellular and Molecular Neurosciencechemistry.chemical_compoundBiological Factorsinhibits DNA synthesisAffinity chromatographyCulture TechniquesmedicineAnimalsRetinaDNA synthesisTissue ExtractsEmbryogenesisEmbryoGeneral MedicineDNATrypsinMolecular biologymedicine.anatomical_structureBiochemistrychemistrySephadexembryonic structuresChromatography GelThymidineCell Divisionmedicine.drugThymidinedescription
Chick embryo retinas contain a peptide factor that inhibits DNA synthesis in explants of chick embryo retina. The inhibitory factor, obtained by acid/ethanol extraction from 15-day-old chick embryo retinas, was partially purified by affinity chromatography on heparin-sepharose CL-6B and gel filtration on Sephadex G-100. The inhibitor reduced DNA synthesis with maximal effects observed in retinal explants from 7 to 8-day-old chick embryos. The inhibitory effect became apparent after 10 h of incubation and reached the maximum levels after 16 h. DNA-inhibiting activity was heat and acid-stable and was destroyed by trypsin and alkaline treatments. The inhibitory effect was observed in retinal explants incubated in a medium free from L-glutamine, and the addition of this compound to the medium reduced the inhibitory effect in a concentration-dependent manner.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-10-01 | Neurochemical research |