6533b873fe1ef96bd12d58e4
RESEARCH PRODUCT
Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP)
Gernot RengerJörg PieperLeonid L. RusevichHarald PaulsenJan Peter EmbsInga Bektassubject
ScatteringPhysicsQC1-999Protein dynamicsDiffusionAnalytical chemistryBuffer solutionSolventCrystallographychemistry.chemical_compoundchemistryQuasielastic neutron scatteringChlorophyll bindingMacromoleculedescription
This study presents quasielastic neutron scattering data of the water-soluble chlorophyll-binding protein (WSCP) and the corresponding buffer solution at room temperature. The contributions of protein and buffer solution to the overall scattering are carefully separated. Otherwise, the fast water dynamics dominating the buffer contribution is likely to mask the slow protein dynamics. In the case of WSCP, the protein scattering can be described by two contributions: i) internal protein dynamics represented by a diffusion in a sphere with an average radius of 2.7 u A and ii) global (Brownian) diffusion of the WSCP macromolecule with an upper limit for the translational diffusion coefficient of 9.4 · 10 −7 cm 2 /s.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-01-01 | EPJ Web of Conferences |