6533b874fe1ef96bd12d60d7
RESEARCH PRODUCT
Real time analysis of Escherichia coli biofilms using calorimetry
Hauke HarmsThomas MaskowBärbel KieselMirko Peitzschsubject
biologyChemistryProcess Chemistry and TechnologyGeneral Chemical EngineeringBiofilmEnergy Engineering and Power TechnologyBiomassGeneral ChemistryCalorimetrybiology.organism_classificationmedicine.disease_causeIndustrial and Manufacturing EngineeringChemical engineeringMicrobial population biologymedicineDegradation (geology)Real time analysisEscherichia coliBacteriadescription
Abstract Microbial communities grow more stably when they are associated to surfaces or organized in aggregates. This advantage of biofilms is technically exploited for the degradation of xenobiotics or in biocatalysis, where the fixed biomass has the added advantage of easier separation of excreted products. Whereas the biothermodynamic analysis of growth and product formation of suspended cells is developing fast, there are only few reports on the calorimetric analysis and biothermodynamic interpretation of biofilm evolution. Experiments illustrate the ability of calorimetry to monitor the physiological state of biofilms in real time. Sessile cells of Escherichia coli DH5α DSM 6897 were selected as surrogates of biofilm formers of medical, ecological and biotechnological importance. It is explained how stoichiometric information can be extracted from calorimetric measurements and future possibilities are discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-06-01 | Chemical Engineering and Processing: Process Intensification |