How Low Can You Go? Low Densities of Poly(ethylene glycol) Surfactants Attract Stealth Proteins.

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RESEARCH PRODUCT

How Low Can You Go? Low Densities of Poly(ethylene glycol) Surfactants Attract Stealth Proteins.

Volker Mailaender Volker Mailaender Anitha Ethirajan Anitha Ethirajan Arthur Ghazaryan Senne Seneca Senne Seneca Claudia Weber Svenja Morsbach Johanna Simon Katharina Landfester

subject

Magnetic Resonance Spectroscopy Polymers and Plastics Nanoparticle Bioengineering Protein Corona 02 engineering and technology 010402 general chemistry 01 natural sciences Polyethylene Glycols nanocarriers; poly(ethylene glycol); protein corona; stealth effect; surfactants Biomaterials chemistry.chemical_compound Colloid Mice Plasma Surface-Active Agents Adsorption PEG ratio Materials Chemistry Animals Humans Colloids Chemistry Sodium Dodecyl Sulfate 021001 nanoscience & nanotechnology 0104 chemical sciences Clusterin RAW 264.7 Cells Chemical engineering Nanoparticles Polystyrenes Protein Corona Adsorption Nanocarriers 0210 nano-technology Ethylene glycol Biotechnology Protein adsorption

description

It is now well-established that the surface chemistry and “stealth” surface functionalities such as poly(ethylene glycol) (PEG) chains of nanocarriers play an important role to decrease unspecific protein adsorption of opsonizing proteins, to increase the enrichment of specific stealth proteins, and to prolong the circulation times of the nanocarriers. At the same time, PEG chains are used to provide colloidal stability for the nanoparticles. However, it is not clear how the chain length and density influence the unspecific and specific protein adsorption keeping at the same time the stability of the nanoparticles in a biological environment. Therefore, this study aims at characterizing the protein adsorption patterns depending on PEG chain length and density to define limits for the amount of PEG needed for a stealth effect by selective protein adsorption as well as colloidal stability during cell experiments. PEG chains are introduced using the PEGylated Lutensol AT surfactants, which allow easy modification of the nanoparticle surface. These findings indicate that a specific enrichment of stealth proteins already occurs at low PEG concentrations; for the decrease of unspecific protein adsorption and finally the colloidal stability a full surface coverage is advised.

year	journal	country	edition	language
2018-06-25	Macromolecular bioscience

10.1002/mabi.201800075 https://pubmed.ncbi.nlm.nih.gov/29943446