Cilvēka dermas mezenhimālo šūnu ilgtermiņa kultūru raksturošana un ilgtermiņa uzglabāšanas optimizēšana

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RESEARCH PRODUCT

Cilvēka dermas mezenhimālo šūnu ilgtermiņa kultūru raksturošana un ilgtermiņa uzglabāšanas optimizēšana

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No cilvēka ādas izdalītas mezenhimālās cilmes šūnas (MCŠ) ir multipotentas šūnas ar potenciālu pielietojumam klīniskajos pētījumos. Lai novērtētu ilgtermiņa in vitro pavairošanas ietekmi uz dermālo MCŠ kultūrām, tika pārbaudīta kariotipa stabilitāte, TERT, diferenciācijas marķieru un ar apoptozi saistītu gēnu ekspresijas izmaiņas pēc 5, 25 un 50 populācijas dalīšanās reizēm (PD). Papildus tika pilnveidota laboratorijā izmantotā ilgtermiņa krioprezervācijas metode. Pēc PD50 šūnas saglabāja normālu kariotipu, neuzsāka TERT ekspresiju, bet pakāpeniski palielinājās apoptozes līmenis kultūrās. Diferenciācijas marķieru ekspresijas panelī tika novērotas ar ar atsevišķām MCŠ kultūrām (iegūtas no atšķirīgiem indivīdiem) saistītas variācijas. Glicerīns tika atzīts par nepiemērotu krioprotektantu ilgtermiņa krioprezervācijā.

year	journal	country	edition	language
2011-01-01

https://dspace.lu.lv/dspace/handle/7/20938

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