Taukaudu mezenhimālo stromālo šūnu iegūšana un raksturošana no SigmaR1 -/- pelēm

6533b874fe1ef96bd12d6666

RESEARCH PRODUCT

Taukaudu mezenhimālo stromālo šūnu iegūšana un raksturošana no SigmaR1 -/- pelēm

Jeļizaveta Krasiļnikova

subject

adipodiferenciācija sigma-1 receptors Farmācija taukaudu stromas šūnas mezenhimālās cilmes šūnas

description

Maģistra darba mērķis bija veikt mezenhimālo cilmes (stromālo) šūnu (MCŠ) izdalīšanu no Sigma-1 receptora (SigmaR1) inaktivētā gēna peļu taukaudiem un noteikt to adipodiferenciācijas potenciālu. Šūnu fenotips tika raksturots, nosakot marķieru ekspresiju ar plūsmas citometriju, savukārt izdalīto šūnu adipodiferenciācija tika noteikta ar attēlu metodi un Oil-Red-O ekstrakciju. Fenotipēšana parādīja, ka lielākā daļa šūnu pieder pie MCŠ, ekspresējot CD29, CD44 un SCA-1 virsmas marķierus virs 90 % populācijas līmenī un daļēji ekspresējot CD105. Adipodiferenciācija uzrādīja statistiski ticamu atšķirību starp kontroles un SigmaR1 inaktivētā gēna (SigmaR1 -/-) grupu, izmantojot Oil-Red-O ekstrakcijas metodi, no kā var secināt, ka inaktivētā gēna šūnām ir samazināta spēja diferencēties par adipocītiem.

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/51287

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