6533b880fe1ef96bd12d6a5d
RESEARCH PRODUCT
Additional file 4: of NOTCH3 expression is linked to breast cancer seeding and distant metastasis
Alexey LeontovichMohammad JalaliradJeffrey SalisburyLisa MillsCandace HaddoxMark SchroederAnn TumaMaria GuicciardiLuca ZammataroMario GambinoAngela AmatoAldo Di LeonardoJames MccubreyCarol LangeMinetta LiuTufia HaddadMatthew GoetzJudy BougheyJann SarkariaLiewei WangJames IngleEvanthia GalanisAntonino Dâassorosubject
description
Figure S4. CRISPR-NOTCH3 breast cancer cells. a NOTCH3 gene knockout using CRISPR/Cas9. Lightning bolt symbols indicate the targeted gene double-stranded break (DSB) sites for different sgRNAs F1 and R2. Horizontal arrows show the PCR primers designed at different chromosomal sites to identify deletions. b A PCR product of ~â 650-bp size is amplified upon a successful double-hit by SRISPR/Cas9 system. c Secondary screening using internal primers. Internal primers were used to screen for clones with efficient gene knockout. Clone 416 was selected for further verification by immunoblot assay (Fig. 4a). (TIFF 6168 kb)
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |