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RESEARCH PRODUCT

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Figure 8. Live human sperm cells in free movement. First frames of a 2 seconds video movies showing: (a) recorded hologram (Visualization 1), (b) pre-filtered hologram (Visualization 2), (c-1)-(c.2) DIC in horizontal (Visualization 3) and vertical (Visualization 4) for the two focusing planes, (d.1)-(d.2) 2D (Visualization 5) and 3D (Visualization 6) quantitative positive phase contrast images for the first focusing plane, and (e.1)-(e.2) 2D (Visualization 7) and 3D (Visualization 8) quantitative negative phase contrast images for the second focusing plane. Scale bars in all images are 50 ���m and lateral gray color bar at images (d.1), (d.2), (e.1) and (e.2) represents optical phase in rad.