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RESEARCH PRODUCT

Additional file 4 of Cellular response to spinal cord injury in regenerative and non-regenerative stages in Xenopus laevis

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Additional file 4: Figure S4. Transgenic line Xla.Tg(Dre.gfap:EGFP)Larra. (A-C) Three different animals’ electroporated in the spinal cord with the CAG promoter driving the expression of EGFP in central canal cells. (D-F) Three different animals electroporated in the spinal cord with the zGFAP::EGFP construct driving specific expression in radial glial like cells in contact with the central canal. (G-J) Animals at different developmental stages of the transgenic line Xla.Tg(Dre.gfap:EGFP)Larra showing expression of EGFP in the neural tube at (G-G’) NF stage 23; (H-H′) NF stage 27; (I-I′) NF stage 31 and in the CNS at (J-J’) NF stage 41. (K-M) Double staining against (K) EGFP and (L) Sox2 in coronal section of the spinal cord at NF stage 43. Panels (M) showed merge image, and panels (M’, M”) are magnifications of the dorsal and ventral cells surrounding the central canal.