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Additional file 5 of The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra

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Additional file 5: Fig. S3. Expression of HAS genes in the interstitial stem cell cluster. (a) t-SNE representation of interstitial cells with clusters labeled by cell state as presented in [25]. (b) Interstitial cell cluster annotation of HyDkk1/2/4 and ataxin genes identified in HyWnt3(+) head lysate fraction. The cells in the t-SNE plots were colored based on expression levels for the respective gene. The transcript IDs are as follows: HMP1: t1098aep, HAS-1: t20535aep, HAS-2: t18494aep, HAS-3: t22149aep, HAS-4: t11453aep, HAS-5: t596aep, HAS-6: t19593aep, HAS-7: t16296aep, HAS-8: t22154aep, HAS-9: t3416aep, HAS-10: t10258aep, HAS-11: t19316aep. HyDkk1/2/4: t8678aep. Cluster label abbreviation key: bat: battery cell, fmgl: female germ-line, gc: gland cell, gmgc: granular mucous gland cell, hyp: hypostome, id: integration doublet, mgl: male germline, nb: nematoblast, nc: neuronal cell, nem: nematocyte, nurse: nurse cells prog: progenitor, SC: stem cell, smgc: spumous mucous gland cell, zmg: zymogen gland cell. Numbers indicate different cell populations within a cluster. (c) Microscopic image showing the epithelial bilayer of the upper gastric region of Hydra. HAS-7 WISH marks gland cells interspersed between the endodermal epithelial cells that are aligned to the central mesoglea (M) separating endo (En)- and ectoderm (Ec). Bar = 20 μM.