6533b882fe1ef96bd12da520

RESEARCH PRODUCT

Additional file 1: Figure S1. of Mycobacterium tuberculosis secretory proteins downregulate T cell activation by interfering with proximal and downstream T cell signalling events

subject

description

(A-C) Lymphocyte transformation test for dose optimisation of Ag85A, ESAT-6 and H37Rv by using PBMCs of healthy individuals (N = 10) and pulmonary TB (N = 10) patients using H3-thymidine uptake assay. PHA and PPD were used as positive control and wells with unstimulated cells were taken as control. Lymphoproliferative responses of healthy individuals and pulmaonary TB patients were calculated using different concentration (2.5, 5, 7.5, 10, 15, μg/ml) of antigens. Bar diagram showing mean ± SEM of stimulation indices (S.I) of stimulated PBMC’s with different doses of PHA, PPD, A85A, ESAT-6 and H37Rv. S.I was calculated according to the formula: S I = Mean counts per minute of experimental wells Mean counts per minute of control wells $$ \mathrm{S}\mathrm{I}=\frac{\mathrm{Mean}\ \mathrm{counts}\ \mathrm{per}\ \mathrm{minute}\ \mathrm{of}\ \mathrm{experimental}\ \mathrm{wells}}{\mathrm{Mean}\ \mathrm{counts}\ \mathrm{per}\ \mathrm{minute}\ \mathrm{of}\ \mathrm{control}\ \mathrm{wells}} $$ (ZIP 160 kb)