6533b882fe1ef96bd12da95b

RESEARCH PRODUCT

Additional file 7 of The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra

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Additional file 7: Fig. S5. Evidence for normal function and morphology of ectopic heads and tentacles. (a-b) Both heads in a HAS-7 siRNA treated animal with a double axis are able to capture and feed on artemia. The arrow denotes an ectopic foot induced by the secondary head. Scale bars = 500 μm. (c-e) Ectopic tentacles induced by ALP treatment show anatomic and molecular features of functional tentacles as demonstrated by immunocytochemistry using a nematocyst-specific antibody (anti-CPP-1) [53]. CPP-1 is a structural component of mature nematocysts in battery cells of tentacles. (c) Overview of CPP-1-stained hydra with ectopic tentacles. Scale bar = 200 μm. (d-e) Enlargement from boxed are in c shows mature stenotele type nematocysts in the ectodermal epithelial layer of the tentacle as visualized by differential interference contrast (d) and corresponding fluorescence image (e). Arrows indicate stenoteles. Scale bars = 20 μm.