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Characterization of an isoproturon mineralizing bacterial culture enriched from a French agricultural soil.
2009
The phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (IPU), was found to be rapidly mineralized by a bacterial culture isolated from an agricultural soil regularly exposed to IPU. Molecular analysis of the bacterial culture by DNA fingerprinting, cloning and sequencing of the 16S rRNA genes revealed that it consisted of six different members among whom the dominant was related to Sphingomonas sp. Six bacterial strains belonging to genera Ancylobacter, Pseudomonas, Stenotrophomonas, Methylobacterium, Variovorax and Agrobacterium were isolated from the IPU-degrading culture. None of these were able to degrade IPU in pure culture and only the intact culture sustained th…
Xenobiotic metabolizing enzyme activities and viability are well preserved in EDTA-isolated rat liver parenchymal cells after cryopreservation
1995
Rat liver parenchymal cells (PC) were isolated by EDTA perfusion and were purified by a subsequent Percoll centrifugation. The isolated PC had a viability of 95%, as judged by trypan blue exclusion. Freshly isolated PC were cryopreserved with an optimized protocol in a computer-controlled freezer. After thawing, the PC still retained a viability of 89%. The activities of representative xenobiotic metabolizing enzymes were compared between freshly isolated and cryopreserved PC after thawing. The cytochrome P450 content and the cytochrome P450 2C11 isoenzyme activity, determined by hydroxylation of testosterone in intact cells, were not affected by the cryopreservation. The following phase II…
Idea novi catalogi critici operum scriptorum hispanorum
Signatures: )(4, A-Z4, Aa2 Alguna capital gravada. - Ornaments tipogràfics Portada a dues tintes. - Reclams
Historia de don Felipe IIII, Rey de las Españas / por don Gonçalo de Cespedes y Meneses...
1634
Sign.:[calderó]4, A-Z8, 2A-2M8, 2N4 Text a 2 col. Port. amb esc. xil. reial
История испанской литературы
1915
История испанской литературы
1915
История испанской литературы
1915
Испанская поэзия в ХѴІ столетии
1915
Испанская поэзия в ХѴІ столетии
1915
El Rey, y por su Magestad Don Luis Guillen de Moncada ... Virrey, y Capitán General del Reyno de Valencia Por quanto la Magestad del Rey ... manda, …
1656
Publicat a València el 10 de febrer de 1656