Search results for " AGI"
showing 10 items of 672 documents
Can Be miR-126-3p a Biomarker of Premature Aging? An Ex Vivo and In Vitro Study in Fabry Disease
2021
Fabry disease (FD) is a lysosomal storage disorder (LSD) characterized by lysosomal accumulation of glycosphingolipids in a wide variety of cytotypes, including endothelial cells (ECs). FD patients experience a significantly reduced life expectancy compared to the general population
Proteomic analysis reveals a role for Bcl2-associated athanogene 3 and major vault protein in resistance to apoptosis in senescent cells by regulatin…
2014
Senescence is a prominent solid tumor response to therapy in which cells avoid apoptosis and instead enter into prolonged cell cycle arrest. We applied a quantitative proteomics screen to identify signals that lead to therapy-induced senescence and discovered that Bcl2-associated athanogene 3 (Bag3) is up-regulated after adriamycin treatment in MCF7 cells. Bag3 is a member of the BAG family of co-chaperones that interacts with Hsp70. Bag3 also regulates major cell-signaling pathways. Mass spectrometry analysis of the Bag3 Complex revealed a novel interaction between Bag3 and Major Vault Protein (MVP). Silencing of Bag3 or MVP shifts the cellular response to adriamycin to favor apoptosis. We…
Vascular aging in rodent models: contrasting mechanisms driving the female and male vascular senescence
2021
Increasing scientific interest has been directed to sex as a biological and decisive factor on several diseases. Several different mechanisms orchestrate vascular function, as well as vascular dysfunction in cardiovascular and metabolic diseases in males and females. Certain vascular sex differences are present throughout life, while others are more evident before the menopause, suggesting two important and correlated drivers: genetic and hormonal factors. With the increasing life expectancy and aging population, studies on aging-related diseases and aging-related physiological changes have steeply grown and, with them, the use of aging animal models. Mouse and rat models of aging, the most…
BCL-xL, a Mitochondrial Protein Involved in Successful Aging: From C. elegans to Human Centenarians
2020
B-Cell Lymphoma-extra-large (BCL-xL) is involved in longevity and successful aging, which indicates a role for BCL-xL in cell survival pathway regulation. Beyond its well described role as an inhibitor of apoptosis by preventing cytochrome c release, BCL-xL has also been related, indirectly, to autophagy and senescence pathways. Although in these latter cases, BCL-xL has dual roles, either activating or inhibiting, depending on the cell type and the specific conditions. Taken together, all these findings suggest a precise mechanism of action for BCL-xL, able to regulate the crosstalk between apoptosis, autophagy, and senescence, thus promoting cell survival or cell death. All three pathways…
Age-associated telomere shortening in mouse oocytes
2013
Abstract Background Oocytes may undergo two types of aging. The first is induced by exposure to an aged ovarian microenvironment before being ovulated, known as ‘reproductive or maternal aging’, and the second by either a prolonged stay in the oviduct before fertilization or in vitro aging prior to insemination, known as ‘postovulatory aging’. However, the molecular mechanisms underlying these aging processes remain to be elucidated. As telomere shortening in cultured somatic cells triggers replicative senescence, telomere shortening in oocytes during reproductive and postovulatory aging may predict developmental competence. This study aimed to ascertain the mechanisms underlying altered te…
Polymer aging affects the bioavailability of microplastics-associated contaminants in sea urchin embryos
2022
Microplastics (MPs) in the marine environment undergo complex weathering factors that can affect their ability to interact with different coexisting environmental contaminants (termed here co-contaminants). In this study, the influence of artificially aging using UV on the sorption of a complex mixture of co-contaminants onto MPs was investigated in order to provide meaningful hypotheses on their individual and combined toxicities on sea urchin embryos. A mixture of artificially aged MPs (PS particles and PA microfibers) combined with 2,2′ ,4,4′ - tetrabromodiphenyl ether (BDE-47), or Cd or Cu, both alone and in a mix, were used to expose embryos of Paracentrotus lividus. The effects of pol…
Experimental evaluation of the reliability of the adhesive joint in a 3rd-generation-solar-cell-integrated glass block
2017
Glass block is a building product that has been widely used since the mid-1800s for the construction of natural light capturing technical elements and, in the last decades, of translucent building envelopes. In a framework of increased quality requirements in terms of energy saving and environmental sustainability, innovations have been introduced to its standard configuration, including the integration of 3rd generation solar cells within the glass block, in order to improve its performance and make it capable of producing clean energy. Indeed, one of the two constituting glass shells has been integrated with a pre-assembled photovoltaic module and then bonded to a plastic “thermal belt” a…
Agile PASSI: An agile process for designing agents
2006
We have been developing robotic multi-agent systems for several years according to a well defined methodology (PASSI) obtaining good results, but day by day needs of a more versatile approach for designing software in a research context suggested us to find out a new methodology. A solution to our problems is represented by the Agile version of the PASSI methodology we present in this paper. We built this agile methodology by exploiting all the experiences done with conventional PASSI; it is supported by specific tools allowing patterns reuse and automatic production of some design documentation.
A metamodelling-based approach for method fragment comparison
2006
Several different approaches to Situational Method Engineering exist. They differ in terms of the primary element of the paradigm: the method fragment definition. Here, we introduce four method fragment definitions from the literature and compare their metamodels according to structural and functional criteria. The structural comparison showed a general alignment of some concepts that are sometimes referred with different names while the study of the compositional aspects results in evidence of substantial differences.
I contratti del minore
2017
L’indagine si propone di risolvere il quesito relativo alla validità o no dei contratti di modico valore conclusi dal minore nella vita quotidiana. Si tenterà di comprendere se essi siano sempre annullabili ex art. 1425 del codice civile italiano, fatta eccezione per l’ipotesi contemplata dall’art. 1426 c.c.; oppure se siano validi, o perché il minore viene considerato nuncius – o, se capace di intendere e di volere, rappresentante volontario – di uno o di entrambi i genitori oppure per applicazione analogica dell’art. 409, comma 2, c.c. Particolare attenzione sarà dedicata ai problemi sollevati all’interprete dai contratti conclusi in via telematica dal minore e alle principali questioni d…