Search results for " Alternative"
showing 10 items of 755 documents
Pharmacogenomic Characterization of Cytotoxic Compounds from Salvia officinalis in Cancer Cells.
2015
Salvia officinalis is used as a dietary supplement with diverse medicinal activity (e.g. antidiabetic and antiatherosclerotic effects). The plant also exerts profound cytotoxicity toward cancer cells. Here, we investigated possible modes of action to explain its activity toward drug-resistant tumor cells. Log10IC50 values of two constituents of S. officinalis (ursolic acid, pomolic acid) were correlated to the expression of ATP-binding cassette (ABC) transporters (P-glycoprotein/ABCB1/MDR1, MRP1/ABCC1, BCRP/ABCG2) and epidermal growth factor receptor (EGFR) or mutations in RAS oncogenes and the tumor suppressor gene TP53 of the NCI panel of cell lines. Gene expression profiles predicting se…
Cytotoxicity of cucurbitacin E from Citrullus colocynthis against multidrug-resistant cancer cells
2019
Abstract Background Cucurbitacin E (CuE) is an oxygenated tetracyclic triterpenoid isolated from the fruits of Citrullus colocynthis (L.) Schrad. Purpose This study outlines CuE's cytotoxic activity against drug-resistant tumor cell lines. Three members of ABC transporters superfamily, P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and ABCB5 were investigated, whose overexpression in tumors is tightly linked to multidrug resistance. Further factors of drug resistance studied were the tumor suppressor TP53 and the epidermal growth factor receptor (EGFR). Methods Cytotoxicity assays (resazurin assays) were used to investigate the activity of Citrullus colocynthis and CuE towar…
Agrobacterium tumefaciens-mediated genetic transformation of the cardenolide-producing plant Digitalis minor L.
2003
A repeatable transformation system has been established for Digitalis minor using Agrobacterium tumefaciens. Leaf explants from 30-day-old seedlings were inoculated with either EHA105 (carrying the nptII and gusA genes) or AGL1 (with the bar and gusA genes) strains. Among the tested factors influencing T-DNA transfer to plants, the EHA105 strain and the addition of acetosyringone to the co-culture medium increased transformation. The highest transformation efficiency (8.4%) was obtained when freshly isolated explants, soaked in a bacterial suspension with an OD 550 of 0.9, were subcultured on selection medium after a 4-day co-culture with the bacteria. Evidence of stable transgene integrati…
Evaluation of Antimicrobial Activity of Triphala Constituents and Nanoformulation
2020
The prevalence of nosocomial infections due to multidrug resistant (MDR) bacterial strains is associated with high morbidity and mortality. Folk medicine and ethnopharmacological data can provide a broad range of plants with promising antimicrobial activity. Triphala, an Ayurvedic formula composed of three different plants: Terminalia chebula Retz., Terminalia bellirica (Gaertn.) Roxb. (Combretaceae), and Phyllanthus emblica L. (Phyllanthaceae), is used widely for various microbial infections. Various extraction techniques were applied in the extraction of the biologically active constituents of Triphala in order to compare their efficiency. Microwave-assisted extraction (MAE) was shown to …
Postural equilibrium: functional system and its stabilization by means of osteoreflexotherapy.
1998
Our investigations have confirmed that the osteoreceptive sensory system participates in the polysensoric regulation of animal and human posture. Experiments with animals have demonstrated that the irritation of osteoreceptors by electric pulses leads to osteoreceptive response potentials of the same parts of the brain (Deiter's nuclei, cerebellum, caudate nucleus, cortical motor centre etc.) which participate in the regulation of animal and human posture and other motion acts. Close functional relations have been established between the skin, bone, vestibular and muscle sensory systems in the brain structures where the osteoreceptive excitation converges. The studies of osteoreceptive sens…
Identifying profiles of complementary and alternative medicine believers and/or users
2020
The present study focuses on the identification of profiles of CAM believers and/or users. Using data from the Spanish CIS Barometer (2018) and a sample of 2486 Spaniards, a hierarchical and non-hierarchical cluster analysis and discriminant analysis have been performed. Profiles of people with a high level of belief in CAM and/or CAM users were identified. Results reflect relationships between personal factors (healthy lifestyle and status, political and religious ideology) and satisfaction with conventional medicine with the belief/use of CAM. Personal factors and satisfaction are not related to the level of belief in CAM. We have tried to fulfil some gaps detected in the literature: much…
Experiences with the use of complementary and alternative medicine in nursing homes: A focus group study.
2016
Abstract Introduction The use of complementary and alternative medicine is increasing outside the Norwegian public health service. The purpose of this study was to gain insight into the experiences of nurses and auxiliary nurses with the use of CAM in care for nursing home residents. Method Focus group interviews with a total of thirteen nurses and auxiliary nurses from three nursing homes were conducted. Data were analyzed using systematic text condensation. Results Participants had experiences from aromatherapy, plant medicine, music therapy and pet therapy. They experienced the use of CAM as effective, exciting and rewarding, but also challenging and dependent on supportive leaders. CAM …
Attitudes About Hypnosis: Factor Analyzing the VSABTH-C With an American Sample
2012
In the present study, the authors factor-analyzed responses from 1,141 American undergraduate students to the Valencia Scale of Attitudes and Beliefs Toward Hypnosis-Client Version. They obtained an 8-factor solution accounting for 66% of the total variance in responses. A confirmatory factor analysis indicated acceptable fit of their model and those reported earlier by Carvalho et al. (2007) and Capafons, Mendoza, et al. (2008) using Portuguese and international samples, respectively. Unlike previous factor analyses of the scale, the authors obtained an independent clusters solution. Distinctions between the authors' model and those reported previously are discussed.
C4, BF, C3 Allele Distribution and Complement Activity in Healthy Aged People and Centenarians
1999
The aim of this study was to examine the complement system and the distribution of some human leukocyte antigen (HLA) class III alleles (C4, BF) in healthy aged people (77 centenarians and 89 elderly subjects). We have also studied the alleles of C3, a complement component genetically unrelated to HLA, the immunochemical levels of C4 and C3 and serum functional hemolytic activity for classical (CH50) and alternative (AP50) complement pathway. The levels of C3 and C4 and the CH50 and AP50 were found to be within the normal range. The frequencies of C3, BF, and C4A alleles were similar in the cohorts that have been studied. For C4B null allele (C4BQ0) a trend toward an increase in the older c…
Interrelationships between dental occlusion and plantar arch
2011
The aim of this study was to evaluate the influence of different jaw relationships on the plantar arch during gait.168 subjects, participating in this study, were distributed into two groups: a control (32 males and 52 females, ranging from 18 to 36 years of age) and a Temporomandibular joint disorders group (28 males and 56 females, ranging from 19 to 42 years of age). Five baropodometric variables were evaluated using a baropodometric platform: the mean load pressure on the plantar surface, the total surface of feet, forefoot vs rearfoot loading, forefoot vs rearfoot surface, and the percentage of body weight on each limb. The tests were performed in three dental occlusion conditions: man…