Search results for " Attachment"

Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector

2008

An ideal gene therapy vector should enable persistent transgene expression without limitations of safety and reproducibility. Here we report the development of a non-viral episomal plasmid DNA (pDNA) vector that appears to fulfil these criteria. This pDNA vector combines a scaffold/matrix attachment region (S/MAR) with a human liver-specific promoter (alpha1-antitrypsin (AAT)) in such a way that long-term expression is enabled in murine liver following hydrodynamic injection. Long-term expression is demonstrated by monitoring the longitudinal luciferase expression profile for up to 6 months by means of in situ bioluminescent imaging. All relevant control pDNA constructs expressing luciferas…

The Influence of Place Attachment, Ad-Evoked Positive Affect, and Motivation on Intention to Visit: Imagination Proclivity as a Moderator

2019

Integrating the theoretical foundations of symbolic interactionism, parasocial interaction, direct affect transfer, push and pull motivational framework, and narrative transportation, this study investigates the determinants of tourists’ intention to visit a destination using Heidi, a famous literary and television series persona, as a stimulus. Place attachment, ad-evoked positive affect, and motivation were theorized as antecedents of intention to visit and imagination proclivity as a moderator. The model was tested using data collected from 410 prospective Spanish tourists. Results show motivation and place attachment as key determinants of intention to visit among individuals with high…

Development of S/MAR minicircles for enhanced and persistent transgene expression in the mouse liver.

2010

We have previously described the development of a scaffold/matrix attachment region (S/MAR) episomal vector system for in vivo application and demonstrated its utility to sustain transgene expression in the mouse liver for at least 6 months following a single administration. Subsequently, we observed that transgene expression is sustained for the lifetime of the animal. The level of expression, however, does drop appreciably over time. We hypothesised that by eliminating the bacterial components in our vectors, we could improve their performance since bacterial sequences have been shown to be responsible for the immunotoxicity of the vector and the silencing of its expression when applied i…

Antecedentes y consecuencias del uso de live videos en el marketing de influencers

2023

Desde la existencia de los medios de comunicación masiva, esto es, prensa, radio, televisión e internet, existen líderes de opinión que influyen en la toma de decisiones de su audiencia. Con la adopción masiva de las plataformas de redes sociales, la ubicuidad que proporcionan los dispositivos móviles y los live videos, la barrera de información entre el líder de opinión y su audiencia se ha reducido significativamente (Carillo et al., 2017). Las empresas, al entender que el desplazamiento de la atención de los consumidores hacia lo digital es más permanente que transitorio, han destinado mayores recursos a la publicidad digital, particularmente, en el influencer marketing o marketing de in…

I hate my workplace but I am very attached to it: workplace attachment style

2017

Purpose The purpose of this paper is to investigate whether different patterns of workplace attachment exist and to explore the relations between adult attachment styles and the level of workplace attachment. Design/methodology/approach Participants were 351 Italian employees who completed a questionnaire composed of the Workplace Attachment Scale and the Relationship Questionnaire. Data were analyzed using correspondence analysis. Findings The results showed that high scores on workplace attachment correlated significantly with secure attachment style, while low scores correlated with insecure attachment styles. These results shed light on different workplace attachment styles. Research l…

Absolute Humidity Influences the Seasonal Persistence and Infectivity of Human Norovirus

2014

ABSTRACT Norovirus (NoV) is one of the main causative agents of acute gastroenteritis worldwide. In temperate climates, outbreaks peak during the winter season. The mechanism by which climatic factors influence the occurrence of NoV outbreaks is unknown. We hypothesized that humidity is linked to NoV seasonality. Human NoV is not cultivatable, so we used cultivatable murine norovirus (MNV) as a surrogate to study its persistence when exposed to various levels of relative humidity (RH) from low (10% RH) to saturated (100% RH) conditions at 9 and 25°C. In addition, we conducted similar experiments with virus-like particles (VLPs) from the predominant GII-4 norovirus and studied changes in bin…

The impact of adult attachment style on organizational commitment and adult attachment in the workplace

2015

International audience; Adult attachment style has only recently been considered as having a role in explaining work behavior. The present research aimed to explore the impact of adult attachment style, assessed by the Adult Attachment Interview (AAI), on organizational commitment (OC) and on adult attachment in the workplace (AAW). We hypothesized that a secure attachment style would be positively related to affective and normative commitment, while preoccupied and avoidant styles would be negatively related to affective commitment; we also hypothesized that there would be a correspondence between the AAI categories and the AAW dimensions. Using the AAI categories as group variable, analys…

Les effets de la vie privée/vie au travail sur l’attachement au travail: le rôle médiateur du fonctionnement interne de l’équipe.

2011

Assessment of the roles of LuxS, Autoinducer 2 and its precursor S-Ribosyl Homocysteine, in cell attachment during biofilm formation by Listeria mono…

2007

No abstract

Terminal tendon cell differentiation requires the glide/gcm complex.

2004

International audience; Locomotion relies on stable attachment of muscle fibres to their target sites, a process that allows for muscle contraction to generate movement. Here, we show that glide/gcm and glide2/gcm2, the fly glial cell determinants, are expressed in a subpopulation of embryonic tendon cells and required for their terminal differentiation. By using loss-of-function approaches, we show that in the absence of both genes, muscle attachment to tendon cells is altered, even though the molecular cascade induced by stripe, the tendon cell determinant, is normal. Moreover, we show that glide/gcm activates a new tendon cell gene independently of stripe. Finally, we show that segment p…