Search results for " Basic"
showing 10 items of 10515 documents
Osallistava sävellytysmenetelmä musiikinopetukseen : peruskoulun opetussuunnitelman 2014 tavoitteita ja oppimisen ydinmetaforia toteuttamassa
2017
In my case study, I used two composing tasks to examine how the inclusive composing method (OSM) I have developed promotes the achievement of the aims of the new core curriculum for basic education 2014 and realises the core metaphors of learning. I have created a learning triangle tool to determine how the musical contents, the principles for developing working culture and the goals of music teaching, transversal competences and national education are linked with the latter. In addition to participation and the gaining and creation of knowledge, I included operative competences in these core metaphors. I examined the means OSM offers to evaluate how the students achieve the aims of musical…
Mucin 1 deficiency mediates corticosteroid insensitivity in asthma
2019
BACKGROUND: The loss of corticosteroid efficacy is an important issue in severe asthma management and may lead to poor asthma control and deterioration of airflow. Recent data indicate that Mucin 1 (MUC1) membrane mucin can mediate corticosteroid efficacy in chronic rhinosinusitis, but the role of MUC1 in uncontrolled severe asthma is unknown. The objective was to analyze the previously unexplored role of MUC1 on corticosteroid efficacy in asthma. METHODS: Mucin 1 expression was evaluated by real-time PCR in human bronchial epithelial cells (HBEC) and blood neutrophils from uncontrolled severe asthma (n=27), controlled mild asthma (n=16), and healthy subjects (n=13). IL-8, MMP9, and GM-CSF …
Discovery of a new class of sortase a transpeptidase inhibitors to tackle gram-positive pathogens: 2-(2-phenylhydrazinylidene)alkanoic acids and rela…
2016
A FRET-based random screening assay was used to generate hit compounds as sortase A inhibitors that allowed us to identify ethyl 3-oxo-2-(2-phenylhydrazinylidene)butanoate as an example of a new class of sortase A inhibitors. Other analogues were generated by changing the ethoxycarbonyl function for a carboxy, cyano or amide group, or introducing substituents in the phenyl ring of the ester and acid derivatives. The most active derivative found was 3-oxo-2-(2-(3,4dichlorophenyl)hydrazinylidene)butanoic acid (2b), showing an IC50 value of 50 µM. For a preliminary assessment of their antivirulence properties the new derivatives were tested for their antibiofilm activity. The most active compo…
Parasite avoidance behaviours in aquatic environments
2018
Parasites, including macroparasites, protists, fungi, bacteria and viruses, can impose a heavy burden upon host animals. However, hosts are not without defences. One aspect of host defence, behavioural avoidance, has been studied in the terrestrial realm for over 50 years, but was first reported from the aquatic environment approximately 20 years ago. Evidence has mounted on the importance of parasite avoidance behaviours and it is increasingly apparent that there are core similarities in the function and benefit of this defence mechanism between terrestrial and aquatic systems. However, there are also stark differences driven by the unique biotic and abiotic characteristics of terrestrial …
Problem Transformation Methods with Distance-Based Learning for Multi-Target Regression
2020
Multi-target regression is a special subset of supervised machine learning problems. Problem transformation methods are used in the field to improve the performance of basic methods. The purpose of this article is to test the use of recently popularized distance-based methods, the minimal learning machine (MLM) and the extreme minimal learning machine (EMLM), in problem transformation. The main advantage of the full data variants of these methods is the lack of any meta-parameter. The experimental results for the MLM and EMLM show promising potential, emphasizing the utility of the problem transformation especially with the EMLM. peerReviewed
MTOR inhibitor-based combination therapies for pancreatic cancer
2018
Background: Although the mechanistic target of rapamycin (MTOR) kinase, included in the mTORC1 and mTORC2 signalling hubs, has been demonstrated to be active in a significant fraction of patients with pancreatic ductal adenocarcinoma (PDAC), the value of the kinase as a therapeutic target needs further clarification. Methods: We used Mtor floxed mice to analyse the function of the kinase in context of the pancreas at the genetic level. Using a dual-recombinase system, which is based on the flippase-FRT (Flp-FRT) and Cre-loxP recombination technologies, we generated a novel cellular model, allowing the genetic analysis of MTOR functions in tumour maintenance. Cross-species validation and pha…
Double copies of blaKPC-3::Tn4401a on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy
2015
ABSTRACT A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla KPC genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla KPC-3 gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla KPC-3 ::Tn 4401a caused by intramolecular transposition events were detected in the plasmid.
The Role of Existential Concerns in the Individual’s Decisions regarding COVID-19 Vaccine Uptake: A Survey among Non-Vaccinated Italian Adults during…
2022
Recent studies have suggested that health constructs embraced by the Terror Management Theory (TMT) and the Basic Psychological Needs Theory (BPNT) may drive individuals’ COVID-19 health-related decisions. This study examines the relationships between existential concerns (ECs; within the TMT), basic psychological needs (BPNs; within the BPNT) and COVID-19 vaccine hesitancy (VH), as well as the mediating role of negative attitudes toward COVID-19 vaccines. A cross-sectional survey was carried out from April to May 2021 on a sample of two hundred and eighty-seven adults (Mage = 36.04 ± 12.07; 59.9% females). Participants provided information regarding existential concerns, basic psychologica…
Aposematism in the burying beetle? Dual function of anal fluid in parental care and chemical defence
2017
Burying beetles (Nicrophorus vespilloides) bear distinctive and variable orange-black patterning on their elytra and produce an anal exudate from their abdomen when threatened. During breeding, the anal exudates contribute to the antimicrobial defence of the breeding resource. We investigated whether the anal exudates also provide a responsive chemical defence, which is advertised to potential avian predators by the beetle’s orange and black elytral markings. We found that that the orange-black elytral markings of the burying beetle are highly conspicuous for avian predators against range of backgrounds, by using computer simulations. Using bioassays with wood ants, we also showed that the …
Colour alone matters : no predator generalization among morphs of an aposematic moth
2018
Local warning colour polymorphism, frequently observed in aposematic organisms, is evolutionarily puzzling. This is because variation in aposematic signals is expected to be selected against due to predators' difficulties associating several signals with a given unprofitable prey. One possible explanation for the existence of such variation is predator generalization, which occurs when predators learn to avoid one form and consequently avoid other sufficiently similar forms, relaxing selection for monomorphic signals. We tested this hypothesis by exposing the three different colour morphs of the aposematic wood tiger moth, Arctia plantaginis, existing in Finland to local wild-caught predato…