Search results for " Basic"
showing 10 items of 10515 documents
Non-redundant and redundant roles of cytomegalovirus gH/gL complexes in host organ entry and intra-tissue spread
2015
Herpesviruses form different gH/gL virion envelope glycoprotein complexes that serve as entry complexes for mediating viral cell-type tropism in vitro; their roles in vivo, however, remained speculative and can be addressed experimentally only in animal models. For murine cytomegalovirus two alternative gH/gL complexes, gH/gL/gO and gH/gL/MCK-2, have been identified. A limitation of studies on viral tropism in vivo has been the difficulty in distinguishing between infection initiation by viral entry into first-hit target cells and subsequent cell-to-cell spread within tissues. As a new strategy to dissect these two events, we used a gO-transcomplemented ΔgO mutant for providing the gH/gL/gO…
Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
2020
Iron is an essential micronutrient for most living beings since it participates as a redox active cofactor in many biological processes including cellular respiration, lipid biosynthesis, DNA replication and repair, and ribosome biogenesis and recycling. However, when present in excess, iron can participate in Fenton reactions and generate reactive oxygen species that damage cells at the level of proteins, lipids and nucleic acids. Organisms have developed different molecular strategies to protect themselves against the harmful effects of high concentrations of iron. In the case of fungi and plants, detoxification mainly occurs by importing cytosolic iron into the vacuole through the Ccc1/V…
Il conto corrente di base tra inclusione finanziaria e controllo della circolazione della ricchezza
2014
Lo scritto prende spunto dalle disposizioni del d.l. 6 dicembre 2011, n. 201, conv. con l. n. 214/2011, introduttive del "contro corrente di base", per ricostruire l'evoluzione, anche su scala sovranazionale, delle politiche di inclusione finanziaria. Esamina quindi le modalità del loro ingresso nel contesto italiano e sottolinea le ambiguità e le insufficienze della neo-introdotta normativa. The essay was inspired by the provisions of Legislative Decree 6 December 2011, n. 201 (conv. in Law Nr. 214/2011), that has introduced tha "basic banking account", and traces the evolution, even on a supranational scale, of the policies on the so-called financial inclusion. The paper then examines the…
Industrial application of selected lactic acid bacteria isolated from local semolinas for typical sourdough bread production
2016
Abstract Four obligate heterofermentative lactic acid bacteria (LAB) strains (Weissella cibaria PON10030 and PON10032 and Leuconostoc citreum PON 10079 and PON10080) were tested as single strain starters, mono-species dual strain starters, and multiple strain starter for the preparation and propagation of sourdoughs for the production of a typical bread at industrial level. The kinetics of pH and TTA during the daily sourdough refreshments indicated a correct acidification process for all trials. The concentration of lactic and acetic acid increased consistently during fermentation. The resulting molar ratios between these two organic acids in the experimental trials were lower than those o…
Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020
2020
8 páginas, 3 figuras
Using UDDI for Publishing Metadata of the Semantic Web
2006
Although UDDI does not provide support for semantic search, retrieval and storage, it is already accepted as an industrial standard and a huge number of services already store their service specifications in UDDI. Objective of this paper is to analyze possibilities and ways to use UDDI registry to allow utilization of meta-data encoded according to Semantic Web standards for semantic-based description, discovery and integration of web resources in the context of needs of two research projects: “Adaptive Services Grid” and “SmartResource”. We present an approach of mapping RDFS upper concepts to UDDI data model using tModel structure, which makes possible to store semantically annotated reso…
The inhibitor of differentiation-1 (Id1) enables lung cancer liver colonization through activation of an EMT program in tumor cells and establishment…
2017
Abstract: Id1 promotes carcinogenesis and metastasis, and predicts prognosis of non-small cell lung cancer (NSCLC)-adenocarcionoma patients. We hypothesized that Id1 may play a critical role in lung cancer colonization of the liver by affecting both tumor cells and the microenvironment. Depleted levels of Id1 in LLC (Lewis lung carcinoma cells, LLC shId1) significantly reduced cell proliferation and migration in vitro. Genetic loss of Id1 in the host tissue (Id1(-/-) mice) impaired liver colonization and increased survival of Id1 animals. Histologically, the presence of Idl in tumor cells of liver metastasis was responsible for liver colonization. Microarray analysis comparing liver tumor n…
A Case of Predation of a Eurasian Eagle-Owl by a Bonelli's Eagle
2016
Although some of these hypotheses are not mutually exclusive, in light of our field observations and experience with both species in the study area, we would favor the first hypothesis as food availability is low in our study area (P. López-López, C. García-Ripollés, J. Giménez, and V. Urios unpubl. data). The second and third hypotheses could also account for this behavior, especially considering previous events of nestling Bonelli's Eagles being killed by eagle-owls (Real and Manosa 1990). Notwithstanding, if the''predatorremoval hypothesis'' were true, the frequency of lethal interactions among both species would be probably much higher and thus previously reported in the literature. Unf…
The elemental role of iron in DNA synthesis and repair
2017
Iron is an essential redox element that functions as a cofactor in many metabolic pathways. Critical enzymes in DNA metabolism, including multiple DNA repair enzymes (helicases, nucleases, glycosylases, demethylases) and ribonucleotide reductase, use iron as an indispensable cofactor to function. Recent striking results have revealed that the catalytic subunit of DNA polymerases also contains conserved cysteine-rich motifs that bind iron–sulfur (Fe/S) clusters that are essential for the formation of stable and active complexes. In line with this, mitochondrial and cytoplasmic defects in Fe/S cluster biogenesis and insertion into the nuclear iron-requiring enzymes involved in DNA synthesis a…
Novel isatin-derived molecules activate p53 via interference with Mdm2 to promote apoptosis
2018
International audience; The p53 protein is a key tumor suppressor in mammals. In response to various forms of genotoxic stress p53 stimulates expression of genes whose products induce cell cycle arrest and/or apoptosis. An E3-ubiquitin ligase, Mdm2 (mouse-double-minute 2) and its human ortholog Hdm2, physically interact with the amino-terminus of p53 to mediate its ubiquitin-mediated degradation via the proteasome. Thus, pharmacological inhibition of the p53-Mdm2 interaction leads to overall stabilization of p53 and stimulation of its anti-tumorigenic activity. In this study we characterize the biological effects of a novel class of non-genotoxic isatin Schiff and Mannich base derivatives (…