Search results for " Bioaerosol"

Exposure to bioaerosols and noise at a Finnish dry waste treatment plant

2004

Repeated measurements were carried out during two different campaigns between 1998 and 2001 to assess the occupational hygiene at a Finnish dry waste treatment plant. The analytical determinations were done in four different places within the processing hall of the plant: near a conveyor belt, near a jigger screen, near an after-crusher and near a bailer. Measurements were also carried out in a coffee room for employees. Concentrations of bacteria, fungi and actinomycetes were determined by two methods (six-stage impactor and Camnea method) and levels of endotoxins, dust and noise were also investigated. High concentrations of microbes and endotoxins and the noise level were found to be a …

2016

Abstract. Bioaerosols pose risks to human health and agriculture and may influence the evolution of mixed-phase clouds and the hydrological cycle on local and regional scales. The availability and reliability of methods and data on the abundance and properties of atmospheric bioaerosols, however, are rather limited. Here we analyze and compare data from different real-time ultraviolet laser/light-induced fluorescence (UV-LIF) instruments with results from a culture-based spore sampler and offline molecular tracers for airborne fungal spores in a semi-arid forest in the southern Rocky Mountains of Colorado. Commercial UV-APS (ultraviolet aerodynamic particle sizer) and WIBS-3 (wideband integ…

High-Resolution Fluorescence Spectra of Airborne Biogenic Secondary Organic Aerosols: Comparisons to Primary Biological Aerosol Particles and Implica…

2021

Aqueous extracts of biogenic secondary organic aerosols (BSOAs) have been found to exhibit fluorescence that may interfere with the laser/light-induced fluorescence (LIF) detection of primary biological aerosol particles (PBAPs). In this study, we quantified the interference of BSOAs to PBAPs by directly measuring airborne BSOA particles, rather than aqueous extracts. BSOAs were generated by the reaction of d-limonene (LIM) or α-pinene (PIN) and ozone (O3) with or without ammonia in a chamber under controlled conditions. With an excitation wavelength of 355 nm, BSOAs exhibited peak emissions at 464–475 nm, while fungal spores exhibited peak emissions at 460–483 nm; the fluorescence intensit…

Metaproteomic analysis of atmospheric aerosol samples.

2016

Metaproteomic analysis of air particulate matter provides information about the abundance and properties of bioaerosols in the atmosphere and their influence on climate and public health. We developed and applied efficient methods for the extraction and analysis of proteins from glass fiber filter samples of total, coarse, and fine particulate matter. Size exclusion chromatography was applied to remove matrix components, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied for protein fractionation according to molecular size, followed by in-gel digestion and LC-MS/MS analysis of peptides using a hybrid Quadrupole-Orbitrap MS. Maxquant software and the Swiss-…

Bioaerosols in the Amazon rain forest: temporal variations and vertical profiles of Eukarya, Bacteria, and Archaea

2021

The Amazon rain forest plays a major role in global hydrological cycling, and biogenic aerosols are likely to influence the formation of clouds and precipitation. Information about the sources and altitude profiles of primary biological aerosol particles, however, is sparse. We used fluorescence in situ hybridization (FISH), a molecular biological staining technique largely unexplored in aerosol research, to investigate the sources and spatiotemporal distribution of Amazonian bioaerosols on the domain level. We found wet season bioaerosol number concentrations in the range of 1–5 × 105 m−3 accounting for > 70 % of the coarse mode aerosol. Eukaryotic and bacterial particles predominated, …

Biotechnology and Conservation of Cultural Heritage

2017

Over the past few decades, biotechnology has provided innovative techniques useful for diagnosing cultural heritage deterioration induced by microbiological systems and for defining efficient conservation/restoration strategies. Seen from this per- spective, the International Congress on Molecular Biology and Cultural Heritage held in Seville (Spain) in 2003 represents a milestone. This book comes from the experience gained in the last decade of basic and applied research, developed in the Laboratory of Biology and Biotechnology for Cultural Heritage (LaBBCH), within the framework of national and international research projects and in collaboration with other Italian and foreign research in…

Occupational hygiene in a Finnish drum composting plant

2005

Bioaerosols (microbes, dust and endotoxins) and volatile organic compounds (VOCs) were determined in the working air of a drum composting plant treating source-separated catering waste. Different composting activities at the Oulu drum composting plant take place in their own units separated by modular design and constructions. Important implication of this is that the control room is a relatively clean working environment and the risk of exposure to harmful factors is low. However, the number of viable airborne microbes was high both in the biowaste receiving hall and in the drum composting hall. The concentration (geometric average) of total microbes was 21.8 million pcs/m3 in the biowaste…

Aerosol Health Effects from Molecular to Global Scales.

2017

Poor air quality is globally the largest environmental health risk. Epidemiological studies have uncovered clear relationships of gaseous pollutants and particulate matter (PM) with adverse health outcomes, including mortality by cardiovascular and respiratory diseases. Studies of health impacts by aerosols are highly multidisciplinary with a broad range of scales in space and time. We assess recent advances and future challenges regarding aerosol effects on health from molecular to global scales through epidemiological studies, field measurements, health-related properties of PM, and multiphase interactions of oxidants and PM upon respiratory deposition. Global modeling combined with epide…

Propuesta de monitorización integral de la calidad ambiental del interior de la Iglesia de los Santos Juanes de Valencia

2020

Para la correcta conservación de los bienes de interés cultural (BIC), debe existir una óptima calidad ambiental interior. La monitorización de esta calidad ambiental, tradicionalmente, incluye el estudio de la humedad y la temperatura de los espacios interiores ya que es bien conocido que sus valores elevados y fluctuaciones rápidas pueden alterar los materiales constitutivos de las obras de arte. Sin embargo, hay muchos otros parámetros que se deben estudiar para tener una visión completa de los riesgos potenciales relacionados con ambientes patrimoniales. En este trabajo, planteamos la importancia de una monitorización integral sistemática de la calidad ambiental de un bien patrimonial q…