Search results for " Biochimica"

The histone deacetylase inhibitor ITF2357 targets oncogenic BRAF in human melanoma cells

2016

ITF2357 (Givinostat) is a potent antineoplastic histone deacetylase inhibitor which is currently used in clinical trials for leukemias and myelomas and in the therapy for systemic juvenile idiopathic arthritis. Here evidence is provided that ITF2357 reduces the viability of human melanoma SK-Mel28 cells thereby inducing cell death. This compound was more efficacious than SAHA, another well known HDAC inhibitor belonging to the same class of hydroxamic acids. Moreover, we demonstrated for the first time that ITF2357 determines in SK-Mel28 cells a remarkable reduction in the level of oncogenic B-Raf, the product of the BRAF V600E mutated gene in melanoma. Western blot analysis showed that the…

Studio dell'autofagia nella citotossicità indotta dai cannabinoidi in cellule tumorali

2013

Autophagy and ER-stress participate to cannabinoid-induced apoptosis in colon carcinoma cells

2012

BIOMARKERS OF OXIDATIVE STRESS AS AN EARLY WARNING SYSTEM TO DETECT IN VITRO XENOBIOTICS’ TOXICITY: EFFECTS OF SUB LETHAL DOSES OF BIS (2-CHLORO-1-ME…

2014

Bioactive compounds from marine resources of western Sicily.

2009

Fed-batch pre-industrial production and purification of a consensus tetratricopeptide repeat (CTPR) scaffold as a container for Fluorescent Proteins …

2022

White light-emitting diodes (WLEDs) are big news in the field of lighting, however, current production processes are still very expensive or based on unsustainable inorganic metals such as inorganic phosphorus. The EU-funded ARTIBLED project aims to produce low-cost and high-efficiency Bio-hybrid light-emitting diodes (Bio-HLEDs). This can be achieved using artificially synthesized fluorescent proteins linked in biological scaffolds like the packaging to obtain LED for lighting applications containing a biogenic phosphor. This study aims to optimize the protein scaffold CTPR10 production process to obtain a high number of scaffolds with a good purity level for Bio-HLEDs construction. Differ…

Evaluation of the in vitro and in vivo antineoplastic effects of Parthenolide on MDA-MB231 breast cancer cells

2012

Triple-negative breast cancer refers to an aggressive subtype of breast cancer in which the tumor cells lack receptors for estrogen, progesterone and the HER2 protein on their surfaces. This type of breast cancer does not respond to treatments such as hormone therapy, like tamoxifen and aromatase inhibitors, or drugs that target HER2, like Herceptin. It is important, therefore, the identification of new selective drugs for the treatment of these tumors. Parthenolide (PN), a sesquiterpene lactone extracted from the medical plant Tanacetum parthenium, exerts anticancer activity on several tumor cell lines in culture, acting through diverse molecular mechanisms. Our previous studies have shown…

IL PARTENOLIDE INDUCE MORTE CELLULARE CASPASI-INDIPENDENTE IN CELLULE DI OSTEOSARCOMA UMANO MG63

2011

FORENSIC INVESTIGATION OF KERATIN MATRIX: DEVELOPMENT OF A METHOD BY TURBOFLOW™ HPLC-MS/MS FOR CANNABINOIDS QUANTITATIVE ANALYSIS

2016

The products of cannabis are the most frequently used illicit drugs of abuse detected in workplace drug-testing programs or in cases of driving under the influence of drugs. For this reason, there is a great need for sensitive and specific methods which can confirm the chronic exposure and which take account of the cut-off values that have been proposed in Italy by the GTFI Guidelines (Group of Italian Forensic Toxicologists). The method currently more appropriate for quantification of Δ9-Tetrahydrocannabinol (THC) and its major metabolite 11-nor-9-Carboxy-Δ9-THC (THCA) appears to be one that involves HPLC-MS/MS and keratin matrix. The purpose of this work, performed at the Mass Spectrometr…

EFFETTI CITOTOSSICI SINERGICI INDOTTI DALLA COMBINAZIONE SAHA/PARTENOLIDE IN CELLULE DI CARCINOMA MAMMARIO UMANO MDA-MB231

Il Partenolide (PN) è un sesquiterpene lattone, estratto dalla pianta medicinale Tanacetum Parthenium, noto per le sue proprietà antiinfiammatorie, antimicrobiche e antitumorali. A tal proposito, recentemente è stato dimostrato che il PN è in grado di esercitare un potente effetto citotossico in cellule di “carcinoma mammario umano triplo negativo” (TNBC) MDA-MB231. Tuttavia quando il composto è impiegato a basse dosi determina effetti citotossici molto limitati, anche dopo lunghi periodi di trattamento. Questi risultati suggeriscono che il PN nelle cellule MDA-MB231 è in grado di indurre effetti citoprotettivi che si evidenziano quando impiegato a basse dosi. Poiché tale caratteristica pot…