Search results for " Biochimica"

PPARa GENE VARIATION AND PHISYCAL PERFORMANCE IN ITALIAN SOCCER PLAYERS

2012

PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα coding for peroxisome proliferator activator receptor alpha, a central regulator of expression of genes involved in fatty acid metabolism. The aim of our research was to study the role of polymorphism of PPARα gene in performance enhancing in Italian soccer players. METHODS: Sixty professional soccer players and thirty sedentary volunteers were enrolled in the study. Samples of venous blood were obtained at rest in the morning by conventional clinical procedures. Serum was collected and lipid profile was measured by using a commercial kit. An aliquote of anticoagulant-treated blood was used to prepare genomic DNA f…

PPARα GENE VARIANTS AS PREDICTED PERFORMANCE ENHANCING POLYMORPHISMS IN PROFESSIONAL ITALIAN SOCCER PLAYERS

2012

PROTEOMIC ANALYSIS OF VESICLES SHED BY BREAST CANCER CELLS IN VITRO

2011

A new method to value efficiency of enzyme blends for pancreas tissue digestion

2010

In pancreatic islets isolation for cell therapy the major enzymes used are obtained from Clostridium hystoliticum: class I and class II collagenases. They are used in a defined tissue dissociation enzyme mixture together with neutral protease (Dispase) or thermolysin (Thermostable Neutral Protease). However, just to now, people working in islets production found variable outcomes in isolation procedures mainly due to large variability in enzymatic blend composition and efficacy. Using electrophoresis and gelatin zymography approaches together with densitometry evaluation assays we compared the composition, stability and auto-digestion processes of C. hystoliticum collagenases, Neutral prote…

Parthenolide induces caspase-independent cell death mediated by AIF in osteosarcoma and melanoma cells.

2012

Parthenolide, the major bioactive sesquiterpene lactone present in Feverfew (Tanacetum parthenium), has recently attracted considerable attention because of its complex pharmacological action involving anti-microbial, anti-inflammatory and anti-cancer effects. However, the mechanism of its cytotoxic effect on tumor cells still remains scarcely defined today. The aim of this study was to analyse the mechanism of parthenolide action on two lines of cancer cells, the human osteosarcoma MG63 and the melanoma SK-MEL-28 cells, on which parthenolide exerted its action inducing similar effects. Staining with Hoechst 33342 showed that parthenolide induced in the first phase of treatment (0-5 h) in m…

Parthenolide induces caspase-independent cell death in osteosarcoma, melanoma and breast cancer cells through the induction of oxidative stress.

2012

Parthenolide, a sesquiterpene lactone found in European feverfew, is used in traditional medicine for its anti-inflammatory activity. In addition, parthenolide has been considered as a novel and effective anti-tumor agent because it induces cytotoxic effects in several tumor cell lines. Our studies demonstrated that parthenolide exerted strong cytotoxic effects in osteosarcoma MG63 and melanoma SK-Mel28 cells in culture. Staining with Hoechst 33342 revealed in most cells after brief periods of treatments (3-5h) chromatin condensation and fragmentation, while only few cells were PI-positive. Prolonging the treatment (5-14h) PI-positive cells strongly augmented, denouncing the increase of nec…

Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes

2015

INTRODUCTION: Post-transcriptional regulation of gene expression relies on RNA-binding proteins (RBPs), which regulate intracellular transport, stability, and translation of mRNAs [1]. We previously identified a set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteins are probably part of a ribonucleoprotein particle [6]. Here we report more details on the expression and intracellular localization of some of these RBPs, during rat brain development and in isolated rat astrocytes. METHODS: Affinity chromatography was performed as already described [6]. Preparation of total lysates and cellular sub-fractions was done as reported in [3]. Possible co-lo…

Plasma Cysteine and Homocysteine concentrations in pregnant and nonpregnant women with controlled folate intake

2010

Purification of nucleoside phosphotransferase from mucosa of chicken intestine

1983

The authors describe the purification procedure and some properties of a nucleoside phosphotransferase obtained from chicken intestinal mucosa, the tissue of which in preliminary studies showed the highest specific activity. Sepharose 6B chromatography and electrophoresis on polyacrylamide gel subdivided the enzyme into many forms which represent various levels of an associated multisubunit protein derived by assembly of a component at lower molecular weight. Nucleotide protectors regulate the equilibrium among these different forms, favoring the production and the stabilization of supramolecular complexes of nucleoside phosphotransferase. Similar results were obtained with enzyme purified …

RB1 in cancer: Different mechanisms of RB1 inactivation and alterations of pRb pathway in tumorigenesis.

2013

Loss of RB1 gene is considered either a causal or an accelerating event in retinoblastoma. A variety of mechanisms inactivates RB1 gene, including intragenic mutations, loss of expression by methylation and chromosomal deletions, with effects which are species-and cell type-specific. RB1 deletion can even lead to aneuploidy thus greatly increasing cancer risk. The RB1gene is part of a larger gene family that includes RBL1 and RBL2, each of the three encoding structurally related proteins indicated as pRb, p107, and p130, respectively. The great interest in these genes and proteins springs from their ability to slow down neoplastic growth. pRb can associate with various proteins by which it …