Search results for " Biochimica"

PCAF catalyzes tha acetylation of spermidine to N8-acetylspermidine and regulates its acetylating activity on histones

2011

Post-transcriptional histone acetylation is a well known process playing a crucial role in chromatin assembly and transcription. Here, we report that PCAF, a highly conserved histone acetyltransferase (HAT), can efficiently catalyze acetylation of spermidine to N8-acetylspermidine, at low concentration. Remarkably, we found that spermidine at higher concentration can also inhibit PCAF HAT activity directed against histone H3 in vitro, confirming the in vivo data referred by Eisenberg et al. on the spermidine induced inhibition of H3 acetylation. Surprisingly when we performed HAT assay experiments at low spermidine concentration we observed an activating effect on PCAF on H3 acetylation. Ou…

Valutazione degli effetti antiossidanti del Resolvis OmegaTM in un modello di disfunzione corneale in vitro.

2015

EPA (acido eicosapentaenoico) e DHA (acido decosaesaeoico) sono derivati metabolici degli AGE (acidi grassi essenziali) e in particolare dell'acido linolenico (omega-3)

Allevamento e qualità del prodotto. In “La stabulazione del tonno rosso mediterraneo (Thunnus thynnus) in Provincia di Trapani”

2009

Combined treatment of caffeic acid and DMAPT induces AMPK dependent death in MDA-MB231 cells

WIN modulates osteosarcoma MG63 cell migration by inhibiting MMPs activity and adjusting intra- and extra-cellular SPARC differential expression

2014

Invasion of cancer cells into surrounding tissue is an initial step in tumor metastasis. This event, which requires migration of cancer cells and attachment to extracellular matrix (ECM), is regulated by elements of the local microenvironment, including ECM architecture. After having demonstrated the ability of the synthetic cannabinoid WIN55,512 to induce osteosarcoma MG63 cell death (1), we studied the effects of WIN on MG63 cell migration. Wound healing assay was performed to measure the ability of cells to migrate and fill the gap obtained by physical disruption of cell monolayer (2). We observed a significant delay in wound closure in 5 M WIN treated cells compared to untreated cells …

The effects of three different training protocols on beginners in eight weeks

2012

The aim of this study was to compare three different methods of training in order to determine the effectiveness on weight loss in relation to body composition in adults. One hundred subjects aged 18 to 38 years, with a BMI equal to or greater than 27 and a body fat percentage greater than 22% for women and 12% for men, were enrolled in this study. They were subdivided into 3 groups that followed a different protocol. Before to start the training protocols was detected the starting level of fitness by a test of maximal oxygen consumption, VO2 max and maximal strength tests that it was detected by one maximal repetition of the major muscle groups; this has allowed to draw up personalized tra…

Flow Cytometry Study of Apoptosis

2008

CAMPTOTHECIN INDUCES S-PHASE ARREST AND APOPTOSIS IN MG63 OSTEOSARCOMA CELLS.

2008

Assessment of biochemical composition of experimental diets for aquaculture, containing plant meals as substitutes for fish meal.

2013

p8 (candidate of metasiasis 1) drives ER-stress/autophagy/apoptosis axis induced by the synthetic cannabinoid WIN in HCC cells.

2010

Background: Today, evidence is emerging for the role of autophagy in the regulation of life and death of tumour cells and its relationship with ER-stress signaling. Our previous results demonstrated that hepatoma HepG2 cells are sensitive to apoptotic effects induced by WIN, a synthetic cannabinoid, which acts through a mechanism involving the reduction in the levels of some survival factors and the activation of pro-apoptotic ones. Since WIN effects were observed after 36−48 hours of treatment, we investigated the possible activation of ER-stress and autophagic process in the first hours of WIN treatment focusing our attention on p8, a factor whose expression is upregulated in response to …